DNA distributions in planktonic bacteria stained with TOTO or TO-PRO

The blue-excitable nucleic acid-binding fluorochromes, TOTO and TO-PRO, were used to stain bacteria in marine plankton communities. Flow cytometric counts of cells stained with these dyes agreed well with counts made by epifluorescence microscopy of DAPI-stained cells. We have used these new dyes in a study of bacteria from various waters in the North Atlantic and the eastern Mediterranean The results indicate a distinction among bacterial groups of different fluorescence intensities (apparent DNA content). At large spatial and temporal scales, about half of the variation in the percentage of bacteria with high apparent DNA content (termed group II bacteria) could be explained by the variation in chlorophyll. Further, the apparent mean DNA content of group II bacteria was also correlated with chlorophyll.