MEASUREMENT OF PLASMA ACETATE KINETICS USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：14

作者：

PERSSON, M ^{[1
]}

BLEIBERG, B ^{[1
]}

KISS, D ^{[1
]}

MILES, J ^{[1
]}

机构：

[1] MAYO CLIN & MAYO FDN,ENDOCRINE RES UNIT,S-164 W JOSEPH,ROCHESTER,MN 55905

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 198卷 / 01期

关键词：

D O I：

10.1016/0003-2697(91)90520-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous studies suggest that plasma acetate may be an important fuel in man, accounting for ∼ 10% of energy expenditure. Available methods for the determination of plasma acetate kinetics are difficult and time consuming. We describe here a procedure for the determination of plasma acetate concentration and specific activity using automated high-performance liquid chromatography that is precise and sensitive and accommodates large numbers of samples. The procedure involves extraction from plasma with diethyl ether, derivatization with bromoacetophenone, and separation on a C-18 reversed-phase column. The specific activities of d-β-hydroxybutyrate and lactate can also be determined. Acetate turnover was measured in four dogs and was similar to that previously reported in sheep and humans. Transport of [14C]acetate into red blood cells was negligible. © 1991.

引用

页码：149 / 153

页数：5

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