From january of 1989 to November of 1991, 25 patients, aged from 19 to 39 years, have been treated using one-stage male to female sex reassignment surgery. The operative technique involved orchiectomy, penectomy, clitoroplasty, urethroplasty, vaginoplasty, pseudocervicoplasty, and vulvoplasty. The main and original procedure of the technique presents formation of a vagina. The new vagina consists of two segments, the vascularized urethral flap and vascularized island tube skin flap, formed from the penile body skin. The urethral segment of the vagina provides moisture to the new vagina. Complications are minimized by such operative technique. Satisfactory anatomic and functional results were achieved in 20(80 percent) of the patients.