GROWTH OF A THERMOTOLERANT ETHANOL-PRODUCING STRAIN OF KLUYVEROMYCES-MARXIANUS ON CELLOBIOSE CONTAINING MEDIA

被引：24

作者：

BARRON, N

MARCHANT, R

MCHALE, L

MCHALE, AP

机构：

[1] Biotechnology Group, School of Applied Biological and Chemical Sciences, University of Ulster, Coleraine, BT52 1SA, Co. Londonderry

来源：

BIOTECHNOLOGY LETTERS | 1994年 / 16卷 / 06期

关键词：

D O I：

10.1007/BF00128612

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The thermotolerant yeast strain, Kluyveromyces marxianus IMB3, was shown to be capable of limited growth on cellobiose containing media at 45 degrees C. Growth, sugar utilization and ethanol production were shown to increase in the presence of exogenously added thermostable fungal beta-glucosidase. During active growth of the organism on cellobiose-containing media, beta-glucosidase activity was detected in cell lysate preparations with only minor amounts of activity present in the extracellular culture filtrate. The results suggest that limitations in ethanol production by this organism during growth on cellobiose containing media may be overcome by addition of exogenously added beta-glucosidase which results in increased substrate access to the biocatalytic unit.

引用

页码：625 / 630

页数：6

共 11 条

[1] OPTIMIZATION OF BATCH PROCESSES INVOLVING SIMULTANEOUS ENZYMATIC AND MICROBIAL REACTIONS [J].

ASENJO, JA ;

SUN, WH ;

SPENCER, JL .

BIOTECHNOLOGY AND BIOENGINEERING, 1991, 37 (11) :1087-1094

[2] ISOLATION OF THERMOTOLERANT, FERMENTATIVE YEASTS GROWING AT 52-DEGREES-C AND PRODUCING ETHANOL AT 45-DEGREES-C AND 50-DEGREES-C [J].

BANAT, IM ;

NIGAM, P ;

MARCHANT, R .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1992, 8 (03) :259-263

[3]

FLEMING M, 1993, BIOTECHNOL LETT, V14, P985

[4] THE CELLULOLYTIC SYSTEM OF TALAROMYCES-EMERSONII - PURIFICATION AND CHARACTERIZATION OF THE EXTRACELLULAR AND INTRACELLULAR BETA-GLUCOSIDASES [J].

MCHALE, A ;

COUGHLAN, MP .

BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 662 (01) :152-159

[5] CELLULASE PRODUCTION DURING GROWTH OF TALAROMYCES-EMERSONII CBS 814.70 ON LACTOSE CONTAINING MEDIA [J].

MCHALE, AP ;

MORRISON, J .

ENZYME AND MICROBIAL TECHNOLOGY, 1986, 8 (12) :749-754

[6] USE OF DINITROSALICYLIC ACID REAGENT FOR DETERMINATION OF REDUCING SUGAR [J].

MILLER, GL .

ANALYTICAL CHEMISTRY, 1959, 31 (03) :426-428

[7] STUDY OF THE ENZYMATIC-HYDROLYSIS OF CELLULOSE FOR PRODUCTION OF FUEL ETHANOL BY THE SIMULTANEOUS SACCHARIFICATION AND FERMENTATION PROCESS [J].

PHILIPPIDIS, GP ;

SMITH, TK ;

WYMAN, CE .

BIOTECHNOLOGY AND BIOENGINEERING, 1993, 41 (09) :846-853

[8] EVALUATION OF THE CELLOBIOSE-FERMENTING YEAST BRETTANOMYCES-CUSTERSII IN THE SIMULTANEOUS SACCHARIFICATION AND FERMENTATION OF CELLULOSE [J].

SPINDLER, DD ;

WYMAN, CE ;

GROHMANN, K ;

PHILIPPIDIS, GP .

BIOTECHNOLOGY LETTERS, 1992, 14 (05) :403-407

[9]

Vallander L., 1990, ADV BIOCHEMICAL ENG, P63, DOI [10.1007/BFb0000731, DOI 10.1007/BFB0000731]

[10]

WYMAN M, 1992, PROCESS BIOCHEM, V27, P239

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