USE OF THE POTASSIUM PYROANTIMONATE-OSMIUM METHOD AS A MEANS OF IDENTIFYING AND LOCALIZING CALCIUM AT THE ULTRASTRUCTURAL LEVEL IN THE CELLS OF CALCIFYING SYSTEMS

被引：59

作者：

APPLETON, J

MORRIS, DC

机构：

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1979年 / 27卷 / 02期

关键词：

D O I：

10.1177/27.2.448059

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Potassium pyroantimonate-osmium was used to localize Ca as an electron dense precipitate in the odontoblast, ameloblast and early hypertrophic chondrocytes of the rat mandibular condylar growth cartilage. The precipitate exhibited a specific association with various cell organelles. The presence of Ca in the precipitates was confirmed using energy dispersive analysis by X-rays. This K-pyroantimonate method apparently provides an accurate technique for the investigation of the subcellular localization of Ca in calcifying systems.

引用

页码：676 / 680

页数：5

共 13 条

[1]

BRIGHTON CT, 1976, FED PROC, V35, P143

[2]

BRIGHTON CT, 1974, CLIN ORTHOP RELAT R, P406

[3] ELECTRON-MICROSCOPIC LOCALIZATION OF CALCIUM IN VASCULAR SMOOTH-MUSCLE [J].