2 ISOFORMS OF THE MUSCLE ACETYLCHOLINE-RECEPTOR ALPHA-SUBUNIT ARE TRANSLATED IN THE HUMAN CELL-LINE TE671

被引：29

作者：

MORRIS, A ^{[1
]}

BEESON, D ^{[1
]}

JACOBSON, L ^{[1
]}

BAGGI, F ^{[1
]}

VINCENT, A ^{[1
]}

NEWSOMDAVIS, J ^{[1
]}

机构：

[1] JOHN RADCLIFFE HOSP,INST MOLEC MED,NEUROSCI GRP,OXFORD OX3 9DU,ENGLAND

来源：

FEBS LETTERS | 1991年 / 295卷 / 1-3期

基金：

英国医学研究理事会;

关键词：

ACHR; ALPHA-SUBUNIT; ISOFORM; TRANSLATION; TE671; CELL;

D O I：

10.1016/0014-5793(91)81399-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have previously reported the existence of 2 forms of mRNA for the human muscle acetylcholine receptor (AChR) alpha-subunit, thought to be generated by alternate splicing of a primary transcript and to encode 2 alpha-subunit protein isoforms [1]. The 2 predicted alpha-subunit isoforms, differing by the insertion of 25 amino acids at position 58/59, have been synthesized from cRNA transcripts using rabbit reticulocyte lysates; these protein isoforms could be differentiated by immunoprecipitation using antibodies raised against synthetic peptides. The antibodies were used to demonstrate translation of both AChR alpha-subunit isoforms in the rhabdomyosarcoma (muscle) cell line TE671, in an approximate 1:1 ratio.

引用

页码：116 / 118

页数：3

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