GERM CELL-SPECIFIC EXPRESSION OF A GENE ENCODING EUKARYOTIC TRANSLATION ELONGATION FACTOR-1-ALPHA (EEF-1-ALPHA) AND GENERATION OF EEF-1-ALPHA RETROPSEUDOGENES IN XENOPUS-LAEVIS

被引：33

作者：

ABDALLAH, B

HOURDRY, J

KRIEG, PA

DENIS, H

MAZABRAUD, A

机构：

[1] UNIV PARIS 11,BIOL DEV VERTEBRES INFERIEURS LAB,CNRS,URA 1134,F-91405 ORSAY,FRANCE

[2] UNIV TEXAS,DEPT ZOOL,CTR DEV BIOL,AUSTIN,TX 78712

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 20期

关键词：

PROCESSED PSEUDOGENES; OOCYTE FORM OF EUKARYOTIC TRANSLATION ELONGATION FACTOR-1-ALPHA;

D O I：

10.1073/pnas.88.20.9277

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have studied by in situ hybridization the expression of the genes encoding the somatic form and the oocyte form of Xenopus laevis eEF-1-alpha. The somatic form of eEF-1-alpha (eEF-1-alpha-S) mRNA is virtually undetectable in male and female germ cells of the adult gonad but is very abundant in embryonic cells after the neurula stage. In contrast, another form of eEF-1-alpha (eEF-1-alpha-O) mRNA is highly concentrated in oogonia and in previtellogenic oocytes but is undetectable in eggs and embryos. eEF-1-alpha-O mRNA is also present in spermatogonia and spermatocytes of adult testis. The latter finding identifies eEF-1-alpha-O mRNA as a germ cell-specific gene product. Although germ cells contain very little eEF-1-alpha-S mRNA, several eEF-1-alpha-S retropseudogenes exist in X. laevis chromosomes. These genes are thought to arise in germ cells from reverse transcription of mRNA and subsequent integration of the cDNA copies into chromosomal DNA. We suggest that eEF-1-alpha-S pseudogenes are generated in primordial germ cells of the embryo before they differentiate into oogonia or spermatogonia.

引用

页码：9277 / 9281

页数：5

共 31 条

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