ABNORMAL MUSCLE DEVELOPMENT IN THE HELDUP3 MUTANT OF DROSOPHILA-MELANOGASTER IS CAUSED BY A SPLICING DEFECT AFFECTING SELECTED TROPONIN-I ISOFORMS

被引：42

作者：

BARBAS, JA ^{[1
]}

GALCERAN, J ^{[1
]}

TORROJA, L ^{[1
]}

PRADO, A ^{[1
]}

FERRUS, A ^{[1
]}

机构：

[1] CSIC,INST CAJAL,CALLE DR ARCE 37,E-28002 MADRID,SPAIN

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1993年 / 13卷 / 03期

关键词：

D O I：

10.1128/MCB.13.3.1433

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The troponin I (TnI) gene of Drosophila melanogaster encodes a family of 10 isoforms resulting from the differential splicing of 13 exons. Four of these exons (6a1, 6a2, 6b1, and 6b2) are mutually exclusive and very similar in sequence. TnI isoforms show qualitative specificity whereby each muscle expresses a selected repertoire of them. In addition, TnI isoforms show quantitative specificity whereby each muscle expresses characteristic amounts of each isoform. In the mutant heldup3, the development of the thoracic muscles DLM, DVM, and TDT is aborted. The mutation consists of a one-nucleotide displacement of the 3' AG splice site at the intron preceding exon 6b1, resulting in the failure to produce all exon 6b1-containing TnI isoforms. These molecular changes in a constituent of the thin filaments cause the selective failure to develop the DLM, DVM, and TDT muscles while having no visible effect on other muscles wherein exon 6b1 expression is minor.

引用

页码：1433 / 1439

页数：7

共 33 条

[1]

ANDREWS LG, 1992, J BIOL CHEM, V267, P7834

[2] TROPONIN-I IS ENCODED IN THE HAPLOLETHAL REGION OF THE SHAKER GENE-COMPLEX OF DROSOPHILA [J].