IN-VIVO ANALYSIS OF STRAIGHT-CHAIN AND BRANCHED-CHAIN FATTY-ACID BIOSYNTHESIS IN 3 ACTINOMYCETES

被引：40

作者：

WALLACE, KK

ZHAO, BT

MCARTHUR, HAI

REYNOLDS, KA

机构：

[1] UNIV MARYLAND,SCH PHARM,DEPT PHARMACEUT SCI,BALTIMORE,MD 21201

[2] PFIZER INC,DIV CENT RES,GROTON,CT 06340

来源：

FEMS MICROBIOLOGY LETTERS | 1995年 / 131卷 / 02期

关键词：

ACTINOMYCETE; BUTYRATE METABOLISM; FATTY ACID BIOSYNTHESIS; AMINO ACID CATABOLISM;

D O I：

10.1111/j.1574-6968.1995.tb07781.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The starter units for branched-chain and straight-chain fatty acid biosynthesis was investigated in vivo in three actinomycetes using stable isotopes. Branched-chain fatty acids, which constitute the majority of the fatty acid pool, were confirmed to be biosynthesized using the amino acid degradation products methylbutyryl-CoA and isobutyryl-CoA as starter units. Straight-chain fatty acids were shown to be constructed using butyryl-CoA as a starter unit. Isomerization of the valine catabolite isobutyryl-CoA was shown to be only a minor source of this butyryl-CoA.

引用

页码：227 / 234

页数：8

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