MOLECULAR WEIGHT OF A PURIFIED CHICKEN ANTIBODY

被引:36
作者
GALLAGHER, JS
VOSS, EW
机构
[1] Department of Microbiology, University of Illinois, Urbana
来源
IMMUNOCHEMISTRY | 1969年 / 6卷 / 02期
基金
美国国家卫生研究院;
关键词
D O I
10.1016/0019-2791(69)90156-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Chicken antibody to the 2,4-DNP grous was purified by an immuneadsorbent. Chemical, physical and immunological studies indicated that this antibody was a single pure component and essentially 100 per cent active. Molecular weight of the antibody calculated from the equation of Svedberg and Pedersen and determined by the high speed equilibrium method of Yphantis was 182,750 and 174,916 respectively. Physical parameters of S20·w, D20·w and partial specific volume were experimentally determined. © 1969.
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页码:199 / +
页数:1
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