PRODUCTION, ISOLATION AND STRUCTURE DETERMINATION OF A NOVEL BETA-GLUCOSIDASE INHIBITOR, CYCLOPHELLITOL, FROM PHELLINUS SP

被引：147

作者：

ATSUMI, S

UMEZAWA, K

IINUMA, H

NAGANAWA, H

NAKAMURA, H

IITAKA, Y

TAKEUCHI, T

机构：

[1] Institute of Microbial Chemistry, Shinagawa-ku, Tokyo 141

[2] Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Kohoku-ku, Yokohama 223, Kanagawa

[3] Laboratory of Biophysics, School of Pharmaceutical Sciences, Teikyo University, Tsukui-gun, 199-01, Kanagawa, Sagamiko-machi

来源：

JOURNAL OF ANTIBIOTICS | 1990年 / 43卷 / 01期

关键词：

D O I：

10.7164/antibiotics.43.49

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In the course of our screening of β-glucosidase inhibitor, a culture filtrate of a mushroom, Phellinus sp. strongly inhibited the enzyme activity. The active substance was isolated through charcoal separation, column chromatography and crystallization. Spectroscopic and crystallographic analysis revealed that it had a novel cyclitol structure, (1S, 2R, 3S, 4R, 5R, 6R)-5-hydroxymethyl-7-oxabicyclo[4,1,0]heptane-2,3,4-triol, and we named it cyclophellitol. It inhibited almond-derived β-glucosidase with an IC50 of 0.8 μg/ml. © 1990, JAPAN ANTIBIOTICS RESEARCH ASSOCIATION. All rights reserved.

引用

页码：49 / 53

页数：5

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