DETERMINATION OF DILTIAZEM HYDROCHLORIDE ENANTIOMERS IN DOG PLASMA USING CHIRAL STATIONARY-PHASE LIQUID-CHROMATOGRAPHY

被引：9

作者：

ISHII, K

BANNO, K

MIYAMOTO, T

KAKIMOTO, T

机构：

[1] Analytical Chemistry Research Laboratory, Tanabe Seiyaku Co., Ltd., Yodogawa-ku, Osaka, 16-89

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 564卷 / 01期

关键词：

D O I：

10.1016/0378-4347(91)80100-Q

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The separation and determination of d- and l-diltiazem hydrochloride in dog plasma by a two-column high-performance liquid chromatographic technique are described. Diltiazem hydrochloride and its metabolites were extracted from dog plasma and analyzed on a conventional column (Nucleosil 5C18) with a volatile buffer system. The column effluent of diltiazem hydrochloride was collected and evaporated. The enantiomeric ratio of the collected diltiazem was determined using a chiral column (Chiralcel OC). The method was accurate and sensitive.

引用

页码：338 / 345

页数：8

共 22 条

[1] USE OF OCTADECASILYL-SILICA FOR EXTRACTION AND PURIFICATION OF PEPTIDES IN BIOLOGICAL SAMPLES - APPLICATION TO IDENTIFICATION OF CIRCULATING METABOLITES OF CORTICOTROPIN-(1-24)-TETRACOSAPEPTIDE AND SOMATOSTATIN INVIVO [J].