METABOLIC ENGINEERING OF MEDICINAL-PLANTS - TRANSGENIC ATROPA-BELLADONNA WITH AN IMPROVED ALKALOID COMPOSITION

被引：167

作者：

YUN, DJ ^{[1
]}

HASHIMOTO, T ^{[1
]}

YAMADA, Y ^{[1
]}

机构：

[1] KYOTO UNIV,FAC AGR,DEPT AGR CHEM,KYOTO 60601,JAPAN

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 24期

关键词：

SCOPOLAMINE; HYOSCYAMINE; 6-BETA-HYDROXYLASE;

D O I：

10.1073/pnas.89.24.11799

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The tropane alkaloid scopolamine is a medicinally important anticholinergic drug present in several solanaceous plants. Hyoscyamine 6beta-hydroxylase (EC 1.14.11.11) catalyzes the oxidative reactions in the biosynthetic pathway leading from hyoscyamine to scopolamine. We introduced the hydroxylase gene from Hyoscyamus niger under the control of the cauliflower mosaic virus 35S promoter into hyoscyamine-rich Atropa belladonna by the use of an Agrobacterium-mediated transformation system. A transgenic plant that constitutively and strongly expressed the transgene was selected, first by screening for kanamycin resistance and then by immunoscreening leaf samples with an antibody specific for the hydroxylase. In the primary transformant and its selfed progeny that inherited the transgene, the alkaloid contents of the leaf and stem were almost exclusively scopolamine. Such metabolically engineered plants should prove useful as breeding materials for obtaining improved medicinal components.

引用

页码：11799 / 11803

页数：5

共 33 条

[1] TOWARD A SCIENCE OF METABOLIC ENGINEERING [J].