AN EXAMINATION OF HEXANE EXTRACT OF FLUE-CURED TOBACCO INVOLVING GEL PERMEATION CHROMATOGRAPHY

被引:10
作者
COOK, CE
TWINE, ME
TALLENT, CR
HARPER, I
HEUNISCH, G
LEWIS, JB
WALL, ME
机构
[1] Chemistry and Life Sciences Laboratory, Research Triangle Institute Research, Triangle Park
关键词
D O I
10.1016/S0031-9422(00)86350-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Techniques have been investigated for separating the hexane extract of flue-cured tobacco into classes based on polarity and molecular size. The method developed, which depends upon counter-current distribution for polarity separation and gel permeation chromatography for molecular size separation, is reproducible, non-destructive, and permits quantitative recovery. Its applicability has been demonstrated by the isolation and identification of several compounds and classes of compounds by following it with conventional chromatographic techniques (column, thin-layer, and gas/liquid chromatography). Among the substances isolated were glycerides, solanesol esters, sterol esters (including cholesterol esters), solanochromene (plastochromenol-8), solanesol, α-tocopherol, β-amyrin, and sterols (including cholesterol). Molecular weight determinations indicated that 70 per cent of the non-basic hexane extract was below 800 in molecular weight. © 1969.
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页码:1025 / &
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