LOCALIZATION AND ENUMERATION OF FIMBRIA-ASSOCIATED ADHESINS OF BACTEROIDES-LOESCHEII

被引:34
作者
WEISS, EI
LONDON, J
KOLENBRANDER, PE
HAND, AR
SIRAGANIAN, R
机构
[1] NIDR, MICROBIOL & IMMUNOL LAB, BETHESDA, MD 20892 USA
[2] NIDR, CLIN INVEST & PATIENT CARE BRANCH, BETHESDA, MD 20892 USA
关键词
D O I
10.1128/jb.170.3.1123-1128.1988
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Monoclonal antibodies that specifically inhibit coaggregation between Bacteroides loescheii PK1295 and its two gram-negative partners Streptococcus sanguis 34 and Actinomyces israelii PK14 were used to enumerate and localize two distinct types of fimbria-associated adhesins on the surface of B. loescheii. Binding studies with radiolabeled monoclonal antibodies indicated that a maximum of (Bmax calculated from Scatchard plots) of approximately 400 adhesin molecules specific for S. sanguis and 310 adhesin molecules specific for A. israelii reside on the surface of the cell. Immunoelectron microscopy revealed that the adhesins were not an integral part of the fimbrial subunit; rather, they were usually found on the distal portion of the structures arranged in a random fashion.
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页码:1123 / 1128
页数:6
相关论文
共 20 条
[1]   MONOCLONAL-ANTIBODIES RAISED AGAINST PAP FIMBRIAE RECOGNIZE MINOR COMPONENT(S) INVOLVED IN RECEPTOR-BINDING [J].
DEREE, JM ;
SCHWILLENS, P ;
VANDENBOSCH, JF .
MICROBIAL PATHOGENESIS, 1987, 2 (02) :113-121
[2]   HOST-SPECIFIC FIMBRIAL ADHESINS OF NON-INVASIVE ENTERO-TOXIGENIC ESCHERICHIA-COLI STRAINS [J].
GAASTRA, W ;
DEGRAAF, FK .
MICROBIOLOGICAL REVIEWS, 1982, 46 (02) :129-161
[3]  
HALES BA, 1986, J GEN MICROBIOL, V132, P2243
[4]   ADHERENCE TO HUMAN COLONOCYTES OF AN ESCHERICHIA-COLI STRAIN ISOLATED FROM SEVERE INFANTILE ENTERITIS - MOLECULAR AND ULTRASTRUCTURAL STUDIES OF A FIBRILLAR ADHESIN [J].
HINSON, G ;
KNUTTON, S ;
LAMPOTANG, MKL ;
MCNEISH, AS ;
WILLIAMS, PH .
INFECTION AND IMMUNITY, 1987, 55 (02) :393-402
[5]  
JONES GW, 1983, CRC CR REV MICROBIOL, V10, P229
[6]  
KLEMM P, 1985, REV INFECT DIS, V7, P321
[7]   MULTIGENERIC AGGREGATIONS AMONG ORAL BACTERIA - A NETWORK OF INDEPENDENT CELL-TO-CELL INTERACTIONS [J].
KOLENBRANDER, PE ;
ANDERSEN, RN .
JOURNAL OF BACTERIOLOGY, 1986, 168 (02) :851-859
[8]   LOCALIZATION OF THE RECEPTOR-BINDING PROTEIN ADHESIN AT THE TIP OF THE BACTERIAL PILUS [J].
LINDBERG, F ;
LUND, B ;
JOHANSSON, L ;
NORMARK, S .
NATURE, 1987, 328 (6125) :84-87
[9]   GLOBOSIDE-SPECIFIC ADHESINS OF UROPATHOGENIC ESCHERICHIA-COLI ARE ENCODED BY SIMILAR TRANS-COMPLEMENTABLE GENE CLUSTERS [J].
LUND, B ;
LINDBERG, FP ;
BAGA, M ;
NORMARK, S .
JOURNAL OF BACTERIOLOGY, 1985, 162 (03) :1293-1301
[10]   ISOLATION AND CHARACTERIZATION OF THE ALPHA-SIALYL-BETA-2,3-GALACTOSYL-SPECIFIC ADHESIN FROM FIMBRIATED ESCHERICHIA-COLI [J].
MOCH, T ;
HOSCHUTZKY, H ;
HACKER, J ;
KRONCKE, KD ;
JANN, K .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (10) :3462-3466