MOLECULAR-CLONING OF AN ALCOHOL (BUTANOL) DEHYDROGENASE GENE-CLUSTER FROM CLOSTRIDIUM-ACETOBUTYLICUM ATCC-824

被引：40

作者：

PETERSEN, DJ ^{[1
]}

WELCH, RW ^{[1
]}

RUDOLPH, FB ^{[1
]}

BENNETT, GN ^{[1
]}

机构：

[1] RICE UNIV,DEPT BIOCHEM & CELL BIOL,HOUSTON,TX 77251

来源：

JOURNAL OF BACTERIOLOGY | 1991年 / 173卷 / 05期

关键词：

D O I：

10.1128/jb.173.5.1831-1834.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In Clostridium acetobutylicum, conversion of butyraldehyde to butanol is enzymatically achieved by butanol dehydrogenase (BDH). A C. acetobutylicum gene that encodes this protein was identified by using an oligonucleotide designed on the basis of the N-terminal amino acid sequence of purified C. acetobutylicum NADH-dependent BDH. Enzyme assays of cell extracts of Escherichia coli harboring the clostridial gene demonstrated 15-fold-higher NADH-dependent BDH activity than untransformed E. coli, as well as an additional NADPH-dependent BDH activity. Kinetic, sequence, and isoelectric focusing analyses suggest that the cloned clostridial DNA contains two or more distinct C. acetobutylicum enzymes with BDH activity.

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页码：1831 / 1834

页数：4

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