IMMUNOPEROXIDASE TECHNIQUE MODIFIED BY COUNTERSTAIN WITH AZURE-B AS A DIAGNOSTIC-AID IN EVALUATING HEAVILY PIGMENTED MELANOCYTIC NEOPLASMS

被引:61
作者
KAMINO, H
TAM, ST
机构
[1] NYU MED CTR,DEPT DERMATOL,DERMATOPATHOL SECT,NEW YORK,NY 10016
[2] NYU MED CTR,DEPT PATHOL,NEW YORK,NY 10016
关键词
D O I
10.1111/j.1600-0560.1991.tb01381.x
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Heavily-pigmented melanocytic neoplasms are difficult to evaluate on routine hematoxylin and eosin stained slides because pigmented melanocytes are difficult to distinguish from the numerous melanophages that are usually seen in the background of these lesions. Immunoperoxidase staining for S 100 protein or HMB-45 antibody using diaminobenzidine (DAB) as chromogen, which forms a brown product, does not adequately distinguish melanocytes from melanophages. We modified this technique by replacing hematoxylin as the counterstain with azure B, which stains melanin green-blue. Thus, positive melanocytes appear brown while melanin granules in their cytoplasm are green-blue. However, negative melanophages only stain green-blue. This technique is useful in evaluating heavily pigmented melanocytic lesions such as malignant melanomas, melanosis of regressing malignant melanoma, residual malignant melanoma in areas of granulation tissue with melanophages, blue nevi, pigmented spindle cell variant of Spitz's nevi and combined nevi.
引用
收藏
页码:436 / 439
页数:4
相关论文
共 17 条
[1]   DISTRIBUTION OF MELANOMA SPECIFIC ANTIBODY (HMB-45) IN BENIGN AND MALIGNANT MELANOCYTIC TUMORS - AN IMMUNOHISTOCHEMICAL STUDY ON PARAFFIN SECTIONS [J].
COLOMBARI, R ;
BONETTI, F ;
ZAMBONI, G ;
SCARPA, A ;
MARINO, F ;
TOMEZZOLI, A ;
CAPELLI, P ;
MENESTRINA, F ;
CHILOSI, M ;
FIOREDONATI, L .
VIRCHOWS ARCHIV A-PATHOLOGICAL ANATOMY AND HISTOPATHOLOGY, 1988, 413 (01) :17-24
[2]   UNIQUE PROTEINS DEFINED BY MONOCLONAL-ANTIBODIES SPECIFIC FOR HUMAN-MELANOMA - SOME POTENTIAL CLINICAL-APPLICATIONS [J].
ESCLAMADO, RM ;
GOWN, AM ;
VOGEL, AM .
AMERICAN JOURNAL OF SURGERY, 1986, 152 (04) :376-385
[3]   A SIMPLE IMMUNOCHEMICAL TECHNIQUE FOR DISTINGUISHING MELANOCYTES AND MELANOPHAGES IN PARAFFIN-EMBEDDED TISSUE [J].
FLEMING, MG ;
BERGFELD, WF .
JOURNAL OF CUTANEOUS PATHOLOGY, 1990, 17 (02) :77-81
[4]  
GOWN AM, 1986, AM J PATHOL, V123, P195
[5]   COLOR MODIFICATION OF DIAMINOBENZIDINE (DAB) PRECIPITATION BY METALLIC-IONS AND ITS APPLICATION FOR DOUBLE IMMUNOHISTOCHEMISTRY [J].
HSU, SM ;
SOBAN, E .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1982, 30 (10) :1079-1082
[6]   THE VALUE OF IMMUNOHISTOCHEMICAL STUDIES USING ANTIBODY TO S100 PROTEIN IN DERMATOPATHOLOGY [J].
KAHN, HJ ;
BAUMAL, R ;
MARKS, A .
INTERNATIONAL JOURNAL OF DERMATOLOGY, 1984, 23 (01) :38-44
[7]   COMPOUND BLUE NEVUS - A VARIANT OF BLUE NEVUS WITH AN ADDITIONAL JUNCTIONAL DENDRITIC COMPONENT - A CLINICAL, HISTOPATHOLOGIC, AND IMMUNOHISTOCHEMICAL STUDY OF 6 CASES [J].
KAMINO, H ;
TAM, ST .
ARCHIVES OF DERMATOLOGY, 1990, 126 (10) :1330-1333
[8]  
LILLIE RD, 1976, HISTOPATHOLOGIC TECH, P132
[9]   IMMUNOPEROXIDASE TECHNIQUES .1. FACTS AND ARTIFACTS [J].
NADJI, M .
AMERICAN JOURNAL OF DERMATOPATHOLOGY, 1986, 8 (01) :32-36
[10]  
NAKAJIMA T, 1982, CANCER, V50, P912, DOI 10.1002/1097-0142(19820901)50:5<912::AID-CNCR2820500519>3.0.CO