PREPARATION OF TETRAHYDROFOLIC ACID AND RELATED-COMPOUNDS BY ELECTROCHEMICAL METHODS

被引：4

作者：

KWEE, S ^{[1
]}

LUND, H ^{[1
]}

机构：

[1] AARHUS UNIV,DEPT ORGAN CHEM,DK-8000 AARHUS C,DENMARK

来源：

BIOELECTROCHEMISTRY AND BIOENERGETICS | 1979年 / 6卷 / 03期

关键词：

D O I：

10.1016/0302-4598(79)80010-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An electrochemical synthesis of 5,6,7,8-tetrahydrofolic acid (H4PteGlu) from folic acid (PteGlu) has been developed. In the first step folic acid is reduced, via 5,8-dihydrofolic acid (5,8-H2PteGlu), to 7,8-H2PteGlu. Reduction of the protonated 7,8-H2PteGlu cleaves C(9)-N(10), but reduction of the unprotonated compound at a more negative potential leads to H4PteGlu in high yield. The reduction is made at relatively low buffer concentration which permits having a neutral pH in the bulk of the solution, but a high pH at the electrode surface due to the electrogenerated base. Besides the preparation of H4PteGlu, the reduction of 5,10-methylidyne-H4PteGlu and 5,10-methylene-H4PteGlu was studied. © 1979.

引用

页码：441 / 450

页数：10