A GENE ENCODING A SUPEROXIDE-DISMUTASE OF THE FACULTATIVE INTRACELLULAR BACTERIUM LISTERIA-MONOCYTOGENES

被引：28

作者：

BREHM, K

HAAS, A

GOEBEL, W

KREFT, J

机构：

来源：

GENE | 1992年 / 118卷 / 01期

关键词：

RECOMBINANT DNA; METALLOENZYME; PARAQUAT; OXIDATIVE STRESS; GENETIC COMPLEMENTATION; PATHOGENIC BACTERIA; HYBRID ENZYME FORMATION;

D O I：

10.1016/0378-1119(92)90258-Q

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A gene (lmsod) encoding superoxide dismutase (SOD; EC 1.15.1.1) of the facultative intracellular pathogen, Listeria monocytogenes, was cloned by functional complementation of an SOD-deficient Escherichia coli mutant. The nucleotide sequence was determined and the deduced amino acid (aa) sequence (202 aa) showed close similarity to manganese-containing SOD's from other organisms. Subunits of the recombinant L. monocytogenes SOD (re-SOD) and of both E. coli SODs formed enzymatically active hybrid enzymes in vivo. DNA/DNA-hybridization experiments showed that this type of recombinant re-sod gene is conserved within the genus Listeria.

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页码：121 / 125

页数：5

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