IMMOBILIZATION OF BETA-GALACTOSIDASE (KLUYVEROMYCES-LACTIS) ON SOLID-PHASE NI(II)-CHELATE

被引：5

作者：

IRAZOQUI, G

BATISTAVIERA, F

机构：

[1] FAC QUIM MONTEVIDEO,CATEDRA BIOQUIM,CASILLA CORREO 1157,MONTEVIDEO,URUGUAY

[2] FAC CIENCIAS MONTEVIDEO,INST QUIM,MONTEVIDEO,URUGUAY

来源：

BIOTECHNOLOGY TECHNIQUES | 1994年 / 8卷 / 04期

关键词：

D O I：

10.1007/BF00155413

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ni(II)-iminodiacetate-agarose achieved a near quantitative adsorption of beta-galactosidase (Kluyveromices lactis) with a yield of 96% of activity applied. A high percentage (90%) of beta-galactosidase activity was preserved after immobilization on the solid phase. The insoluble derivative could be used for lactose hydrolysis either in solution or in whey permeate.

引用

页码：233 / 238

页数：6

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