ION-TRANSLOCATING ATPASES IN TENDRILS OF BRYONIA-DIOICA JACQ

被引:33
作者
LISS, H [1 ]
WEILER, EW [1 ]
机构
[1] RUHR UNIV BOCHUM,LEHRSTUHL PFLANZENPHYSIOL,D-44780 BOCHUM,GERMANY
关键词
BRYONIA; CA2+-ATPASE; ENDOPLASMIC RETICULUM; PLASMALEMMA; TENDRIL COILING;
D O I
10.1007/BF01101675
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Procedures have been developed which allow the preparation of highly pure endoplasmic reticulum and plasma membrane from tendrils of Bryonia dioica. These and further membrane fractions were used to study vanadate-sensitive ATPase activity as well as Mg2+-ATP-driven transport of Ca-45(2+). Calcium-translocating ATPases were detected in the endoplasmic reticulum, the plasma membrane and the mitochondrial fraction and characterized kinetically and with respect to the effects of various inhibitors. The endoplasmic-reticulum Ca2+-translocating ATPase was stimulated by KCl and was calmodulin-dependent. The plasma-membrane enzyme was not affected by these agents. These, as well as the inhibitor data, show that the Ca2+-translocating ATPases of the endoplasmic reticulum and the plasma membrane are distinctly different enzymes. Upon mechanical stimulation, the activities of the vanadate-sensitive K+, Mg2+-ATPase and the Ca2+-translocating ATPase(s) increased rapidly and transiently, indicating that increasing transmembrane proton and calcium fluxes are involved in the early stages of tendril coiling.
引用
收藏
页码:169 / 180
页数:12
相关论文
共 40 条
[1]   COPPER ENZYMES IN ISOLATED CHLOROPLASTS - POLYPHENOLOXIDASE IN BETA-VULGARIS [J].
ARNON, DI .
PLANT PHYSIOLOGY, 1949, 24 (01) :1-15
[2]   RECONSTITUTION AND CHARACTERIZATION OF A CALMODULIN-STIMULATED CA-2+-PUMPING ATPASE PURIFIED FROM BRASSICA-OLERACEA L [J].
ASKERLUND, P ;
EVANS, DE .
PLANT PHYSIOLOGY, 1992, 100 (04) :1670-1681
[3]  
BENEDETTO C, 1987, PROSTAGLANDINS RELAT, P29
[4]   IMPROVED SILVER STAINING OF PLANT-PROTEINS, RNA AND DNA IN POLYACRYLAMIDE GELS [J].
BLUM, H ;
BEIER, H ;
GROSS, HJ .
ELECTROPHORESIS, 1987, 8 (02) :93-99
[5]   MICROSOMAL ATP-DEPENDENT CA-2+ TRANSPORT AS AFFECTED BY ENVIRONMENTAL-STRESS IN BRYONIA-DIOICA INTERNODES [J].
BOURGEADE, P ;
DEJAEGHER, G ;
BOYER, N .
PLANT SCIENCE, 1991, 79 (01) :23-30
[6]   RAIN-INDUCED, WIND-INDUCED, AND TOUCH-INDUCED EXPRESSION OF CALMODULIN AND CALMODULIN-RELATED GENES IN ARABIDOPSIS [J].
BRAAM, J ;
DAVIS, RW .
CELL, 1990, 60 (03) :357-364
[7]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[8]   CA-2+-TRANSLOCATING ATPASE OF THE PLANT PLASMA-MEMBRANE [J].
BRISKIN, DP .
PLANT PHYSIOLOGY, 1990, 94 (02) :397-400
[9]  
CARAFOLI E, 1992, J BIOL CHEM, V267, P2115
[10]   A PLASMA MEMBRANE-TYPE CA-2+-ATPASE OF 120 KILODALTONS ON THE ENDOPLASMIC-RETICULUM FROM CARROT (DAUCUS-CAROTA) CELLS - PROPERTIES OF THE PHOSPHORYLATED INTERMEDIATE [J].
CHEN, FH ;
RATTERMAN, DM ;
SZE, H .
PLANT PHYSIOLOGY, 1993, 102 (02) :651-661