ACETYLCHOLINE-RELEASE FROM DISSOCIATED STRIATAL CELLS

被引：10

作者：

BORLAND, K ^{[1
]}

HARRISON, MB ^{[1
]}

RAGOZZINO, ME ^{[1
]}

GOLD, PE ^{[1
]}

机构：

[1] UNIV VIRGINIA, DEPT PSYCHOL, CHARLOTTESVILLE, VA 22908 USA

来源：

BRAIN RESEARCH | 1995年 / 697卷 / 1-2期

关键词：

ACETYLCHOLINE; CALCIUM; DISSOCIATED CELL; FRACTIONAL EFFLUX; GLUTAMATE; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; STRIATUM;

D O I：

10.1016/0006-8993(95)00996-4

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

To study the regulation of striatal acetylcholine (ACH) release, adult male rat striata were dissociated and incubated with 3H-choline to synthesize 3H-ACH. Fractional 3H-ACH efflux per min during continuous perifusion was: (1) tightly regulated; (2) dependent on calcium influx; (3) stimulated by 10 mM K+ and 1 mM glutamate; and (4) comparable to ACH release detected by HPLC. Thus, acutely dissociated striata exhibit calcium-sensitive, voltage-dependent secretion of 3H-ACH and direct receptor-mediated stimulation of release through the glutamate receptor family. This new approach toward cholinergic secretory physiology will help clarify complex striatal circuitry.

引用

页码：271 / 275

页数：5

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