AGGREGATION OF MAMMALIAN-CELLS EXPRESSING THE PLATELET GLYCOPROTEIN (GP) IB-IX COMPLEX AND THE REQUIREMENT FOR TYROSINE SULFATION OF GP IB-ALPHA

The glycoprotein (GP) Ib-IX complex mediates platelet aggregation in response to high shear forces by binding von Willebrand factor (VWF) in the plasma. We investigated the possibility that the complex could mediate a similar phenomenon if expressed in nonhematopoietic cells. When agitated on a tabletop shaker, CHO and L cells expressing the full complex formed large aggregates in the presence of VWF and the modulator ristocetin. When the rate of agitation was increased, aggregation occurred without added ristocetin and appeared to require only the application of a physical force. The aggregation was homophilic and temperature-dependent and required a functional ligand-binding subunit of the GP Ib-IX complex, GP Iba. Posttranslational tyrosine sulfation of GP Ib alpha was required for aggregate formation and stability. Thus, aggregation of cells expressing the GP Ib-IX complex is a unique example of a ligand-receptor interaction induced by mechanical forces and demonstrates an important biological role for sulfation of tyrosine residues. (C) 1995 by The American Society of Hematology.