GLUCOSE BIOSENSOR BASED ON IMMOBILIZATION OF GLUCOSE-OXIDASE IN AN ANIONIC ION-EXCHANGE POLYMER BLEND

被引：6

作者：

FORTIER, G ^{[1
]}

BELANGER, D ^{[1
]}

CASAVANT, L ^{[1
]}

WILSON, C ^{[1
]}

LAWRENCE, MF ^{[1
]}

机构：

[1] CONCORDIA UNIV,DEPT CHEM,MONTREAL H3G 1M8,QUEBEC,CANADA

来源：

ANALYTICAL LETTERS | 1992年 / 25卷 / 10期

基金：

加拿大自然科学与工程研究理事会;

关键词：

BIOSENSOR; GLUCOSE OXIDASE; AMPEROMETRIC; POLYXIO; POLY[(4-VINYLPYRIDINE)-CO-STYRENE; QUATERNIZED STYRENE POLYMER;

D O I：

10.1080/00032719208018256

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A fast and simple procedure to prepare a glucose oxidase electrode is presented herein. A blend of poly[(4-vinylpyridine)-co-styrene] and a polystyrene with quaternized amine groups, named PolyXIO, solubilized in methanol has been used for the immobilization of glucose oxidase at the surface of a platinum electrode. The response of the biosensor is saturated at 20 mM of glucose and a linearity of current versus glucose concentration is observed up to 4 mM. With 5U of GOD entrapped in the polymer membrane, the maximum current reached 200 muA/cm2. The major advantages of this polymer blend lie in its good ionic diffusion capability, and because it has pendant groups opposite in charge to the outer groups on glucose oxidase.

引用

页码：1835 / 1842

页数：8

共 11 条

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