DETECTION OF THE GERMAN GRAPEVINE YELLOWS (VERGILBUNGSKRANKHEIT) MLO IN GRAPEVINE, ALTERNATIVE HOSTS AND A VECTOR BY A SPECIFIC PCR PROCEDURE

被引：233

作者：

MAIXNER, M ^{[1
]}

AHRENS, U ^{[1
]}

SEEMULLER, E ^{[1
]}

机构：

[1] BIOL BUNDESANSTALT LAND & FORTSWIRTSCHAFT,INST PFLANZENSCHUTZ OBSTBAU,D-69216 DOSSENHEIM,GERMANY

来源：

EUROPEAN JOURNAL OF PLANT PATHOLOGY | 1995年 / 101卷 / 03期

关键词：

DIAGNOSIS; MOLLICUTES; EPIDEMIOLOGY; TRANSMISSION; PLANTHOPPER;

D O I：

10.1007/BF01874780

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A polymerase chain reaction procedure was developed which enables specific amplification of a ribosomal sequence from the mycoplasmalike organism (MLO) associated with German grapevine yellows (Vergilbungskrankheit, VK) and stolbur-related diseases of solanaceous plants. Successful amplification from all samples prepared from various cultivars collected in different viticultural areas indicates that the causal agent is a relatively homogeneous organism. Amplification was also achieved with template DNA prepared from naturally infected weeds in vineyards such as Convolvolus arvensis and Solanum nigrum, and from the planthopper Hyalesthes obsoletus that was collected in the vineyards. Feeding of insects of this species on grapevine seedlings resulted in the development of typical yellows symptoms by the grapes. H. obsoletus could therefore be identified as a vector of Vergilbungskrankheit.

引用

页码：241 / 250

页数：10

共 27 条

[1]

Ahrens U., Seemuller E., Detection of DNA of plant pathogenic mycoplasmalike organisms by a polymerase chain reaction that amplifies a sequence of the 16S rRNA gene, Phytopathology, 82, pp. 828-832, (1992)

[2]

Bianco P.A., Davis R.E., Prince J.P., Lee I.M., Gundersen D.E., Fortusini A., Belli G., Double and single infections by aster yellows and elm yellows MLOs in grapevines with symptoms characteristic of flavescence dorée, Riv. Pat. Veg., S.V., 3, pp. 69-82, (1993)

[3]

Caudwell A., Gianotti J., Kuszala C., Larrue J., Etude du role de particules de type Mycoplasme dans l'etiologique de la flavescence dorée de la vigne. Examen cytologique des plantes malades et des cicadelles infectieuses, Ann Phytopathol, 3, pp. 107-123, (1971)

[4]

Caudwell A., Larrue J., Kuszala C., Bachelier J.C., Pluralité des jaunisses de la vigne, Ann Phytopathol, 3, pp. 95-105, (1971)

[5]

Chen K.H., Guo J.R., Wu X.Y., Loi N., Carraro L., Guo Y.D., Osler R., Pearson R., Chen T.A., Comparison of monoclonal antibodies, DNA probes, and PCR for detection of the grapevine yellows disease agent, Phytopathology, 83, pp. 915-922, (1993)

[6]

Credi R., Callegari D., Profilo epidemiologico della flavescenza dorata della vite in Emilia Romagna: difussione temporale, distribuzione spaziale delle piante ammalate e gradienti d'incidenza, Phytopat medit, 27, pp. 90-98, (1988)

[7]

Daire X., Boudon-Padieu E., Berville A., Schneider B., Caudwell A., Cloned DNA probes for detection of grapevine flavescence dorée mycoplasma-like organism (MLO), Annals of Applied Biology, 121, pp. 95-103, (1992)

[8]

Daire X., Clair D., Larrue J., Boudon-Padieu E., Caudwell A., Diversity among mycoplasma-like organisms inducing grapevine yellows in France, Vitis, 32, pp. 159-163, (1993)

[9]

Daire X., Clair D., Larrue J., Boudon-Padieu E., Alma A., Arzone A., Carraro L., Osler R., Refatti E., Granata G., Credi R., Tanne E., Pearson R., Caudwell A., Occurrence of diverse MLOs in tissues of grapevine affected by grapevine yellows in different countries, Vitis, 32, pp. 247-248, (1993)

[10]

Davis R.E., Bertaccini A., Prince J.P., Vibio M., Infection of grapevines in Emilia-Romagna by mycoplasmalike organisms (MLOs) related to Italian periwinkle virescence MLO: evidence from enzymatic amplification of MLO DNA, Phytopath medit, 32, pp. 149-152, (1993)

← 1 2 3 →