INFLUENCE OF GROWTH PHASE AND FIMBRIAE ON GRADIENT ELUTION OF SALMONELLA-TYPHIMURIUM FROM DEAE-CELLULOSE

被引：7

作者：

HALL, AN

HOGG, SD

PHILLIPS, GO

机构：

[1] Department of Chemistry and Applied Chemistry, University of Salford, Salford

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1978年 / 44卷 / 02期

关键词：

D O I：

10.1111/j.1365-2672.1978.tb00793.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Initial investigations showed that certain populations of Sytyphimurium cells could be resolved into three fractions by eluting the cells from DEAE‐cellulose with a NaCl gradient in 0·05 Mphosphate buffer (pH 6·0). The three fractions were eluted with 0, 60 and 190 mmol/l NaCl. The results obtained with two strains indicate that non‐fimbriate, exponential phase cells; fimbriate, stationary phase cells; and non‐fimbriate, stationary phase cells are eluted with 0, 60 and 190 mmol/l NaCl in 0·05 M‐phosphate buffer (pH 6·0), respectively. These conclusions appear to be consistent with current views on the effects of fimbriae and the exponential growth phase in reducing the electrical charge carried by bacterial cells. Copyright © 1978, Wiley Blackwell. All rights reserved

引用

页码：215 / 223

页数：9