A SYNTHETIC PEPTIDE CORRESPONDING TO RESIDUE-137 TO RESIDUE-157 OF P60V-SRC INHIBITS TYROSINE-SPECIFIC PROTEIN-KINASES

被引：27

作者：

SATO, K

MIKI, S

TACHIBANA, H

HAYASHI, F

AKIYAMA, T

FUKAMI, Y

机构：

[1] KOBE UNIV,BIOSIGNAL RES CTR,MOLEC BIOL LAB,NADA KU,KOBE 657,JAPAN

[2] KOBE UNIV,FAC SCI,DEPT BIOL,KOBE 657,JAPAN

[3] KOBE UNIV,FAC GEN EDUC,DEPT BIOL,KOBE 657,JAPAN

[4] OSAKA UNIV,MICROBIAL DIS RES INST,SUITA,OSAKA 565,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 171卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)90805-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 21-residue synthetic peptide corresponding to a part of the noncatalytic domain of p60v-src (residues 137 to 157) was found to inhibit the tyrosine kinase activity of p60v-src. The half inhibition concentration was ca. 7.5 μM. The peptide (peptide A) did not compete with substrate proteins or ATP. Peptide A also inhibited the autophosphorylation of epidermal growth factor receptor/kinase and the tyrosine-specific protein phosphorylation in the acetylcholine receptor-rich membranes isolated from electroplax of Narke japonica. However, serine/threonine-specific protein kinases such as cAMP-dependent and cGMP-dependent protein kinases were not inhibited by peptide A. © 1990.

引用

页码：1152 / 1159

页数：8

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