INTRODUCTION AND EXPRESSION OF A RABBIT BETA-GLOBIN GENE IN MOUSE FIBROBLASTS

被引：114

作者：

WOLD, B

WIGLER, M

LACY, E

MANIATIS, T

SILVERSTEIN, S

AXEL, R

机构：

[1] COLD SPRING HARBOR LAB,COLD SPRING HARBOR,NY 11724

[2] CALTECH,DIV BIOL,PASADENA,CA 91125

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1979年 / 76卷 / 11期

关键词：

D O I：

10.1073/pnas.76.11.5684

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The cloned chromosomal rabbit beta-globin gene has been introduced into mouse fibroblasts by DNA-mediated gene transfer (transformation). In this report, we examine the expression of the rabbit gene in six independent transformants that contain from 1 to 20 copies of the cloned globin gene. Rabbit globin transcripts were detected in two of these transformants at steady-state concentrations of 5 and 2 copies per cell. The globin transcripts from one cell line are polyadenylylated and migrate as 9S RNA on methylmercury gels. These transcripts reflect correct processing of the two intervening sequences but lack 48 +/- 5 nucleotides present at the 5' terminus of rabbit erythrocyte globin mRNA.

引用

页码：5684 / 5688

页数：5

共 25 条

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