EFFECT OF SODIUM DEOXYCHOLATE ON THE EXTRACTION OF MYOCARDIAL CREATINE-PHOSPHOKINASE AND PROTEIN

Rat heart creatine phosphokinase (EC 2.7, 3.2) was extracted in the presence and absence of varying concentrations of sodium desoxycholate and the enzyme activity and total protein content of the extract was determined. Utilization of 0.05 and 0.10% desoxycholate had no effect on the extraction of creatine phosphokinase, while it caused a 70-75% increase in the protein value. Concentrations of 0.5, 0.75 and 1.0% desoxycholate almost doubled the release of creatine phosphokinase. Concomitantly, protein values rose five-to sevenfold. It is suggested that the addition of sodium desoxycholate to a concentration of 0.5% will greatly enhance myocardial creatine phosphokinase release provided the protein content of the extract is not desired as a reference point. © 1979.