CHEMILUMINESCENT DETERMINATION OF CLINICALLY IMPORTANT ORGANIC REDUCTANTS

The chemiluminescent (CL) reaction of lucigenin with reducing agents in alkaline solution has been investigated for determination of certain clinically Important organic species. Detection limits (in mg/L) were found to be: ascorbic acid, 0.17; creatinine, 4.7; uric acid, 0.64; glutathione, 1.0; glucuronic acid, 9.1; lactose, 5.2; glucose, 21. The relative standard deviation of the CL signal is in the range of 0.5-5 %. Certain metal ions (Fe(III), Mn(II), Cu(II)) interfere with the determination. The observed emission Intensity for a mixture of analytes is equal to the sum of the emission intensities for the separate analytes. © 1979, American Chemical Society. All rights reserved.