PREPARATION TECHNIQUES FOR THE ELECTRON-MICROSCOPY OF GRANULAR SLUDGE FROM AN ANAEROBIC DIGESTER

被引：6

作者：

HOWGRAVEGRAHAM, AR

WALLIS, FM

机构：

[1] Department of Microbiology and Plant Pathology, University of Natal, Pietermaritzburg, 3200

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1991年 / 13卷 / 02期

关键词：

D O I：

10.1111/j.1472-765X.1991.tb00577.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Several fixation and dehydration techniques for scanning and transmission electron microscopy of glycocalyx and microbial populations within granules from an upflow anaerobic sludge blanket digester purifying a brewery wastewater were compared. Sputter-cryo and freeze-drying techniques prior to scanning electron microscopy (SEM) allowed viewing of the glycocalyx. In contrast standard fixation and dehydration techniques were suitable for examination of underlying microbial populations by both SEM and transmission electron microscopy.

引用

页码：87 / 89

页数：3

共 10 条

[1]

[Anonymous], [No title captured]

[2] THE BACTERIAL GLYCOCALYX IN NATURE AND DISEASE [J].