IMPACT OF ANTIGEN-PRESENTING CELLS ON CYTOKINE PROFILES OF HUMAN TH CLONES ESTABLISHED AFTER STIMULATION WITH MYCOBACTERIUM-TUBERCULOSIS ANTIGENS

被引：17

作者：

CONRADT, P

KAUFMANN, SHE

机构：

来源：

INFECTION AND IMMUNITY | 1995年 / 63卷 / 05期

关键词：

D O I：

10.1128/IAI.63.5.2079-2081.1995

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Human T cells reactive with mycobacterial antigens are generally considered to correlate with a Th1 cytokine profile. Our data show that, in addition, Th0 and Th2 clones develop in bulk culture with appropriate antigen-presenting cells before cloning. CD4(+) blasts activated by mycobacterial antigens were cloned, and their mRNA patterns for the interleukins (IL) IL-2, IL-4, IL-5, IL-6, and IL-10 and gamma interferon were characterized by reverse-transcribed PCR. Nonadherent, nonrosetting, enriched peripheral blood mononuclear cells promoted development of Th0; after further depletion of monocytes and natural killer cells? Th2 clones were also found, Epstein-Barr virus-transformed B cells, with specificity for the stimulating antigen, increased the proportion of Th2 clones.

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页码：2079 / 2081

页数：3

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