TANNIN PRODUCTION IN PLANT-CELL CULTURES .3. CONDENSED TANNIN PRODUCTION IN CALLUS AND SUSPENSION-CULTURES OF CINNAMOMUM-CASSIA

被引:17
作者
YAZAKI, K [1 ]
OKUDA, T [1 ]
机构
[1] OKAYAMA UNIV,FAC PHARMACEUT SCI,OKAYAMA 700,JAPAN
关键词
callus culture; Cinnamomum cassia; condensed tannin; Lauraceae; plant cell culture; procyanidins; suspension culture;
D O I
10.1016/0031-9422(90)80121-V
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Callus and suspension cultures of Cinnamomum cassia were found to produce a large amount of condensed tannin. ( - )-Epicatechin and procyanidins B2, B4 and C1 which are precursors of condensed tannin were isolated from callus cultures. Tannin production in callus cultures was stimulated by coconut milk or malt extract supplemented in the medium. In suspension cultures, cell growth in Linsmaier-Skoog's medium containing coconut milk was better than that in the absence of coconut milk. The time-course of cell growth and tannin content showed that the production of procyanidin dimer increased in the long lag phase of callus cultures. © 1990.
引用
收藏
页码:1559 / 1562
页数:4
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