21-PERCENT PARTIAL-HEPATECTOMY - INVIVO RAT MODEL FOR THE STUDY OF LIVER-REGENERATION

被引:2
作者
BARUCH, Y
FRITH, LO
KIRSCH, RE
机构
[1] TECHNION ISRAEL INST TECHNOL,FAC MED,IL-31096 HAIFA,ISRAEL
[2] UNIV CAPE TOWN,SCH MED,OBSERV,CAPE TOWN,SOUTH AFRICA
关键词
D O I
10.1016/0168-8278(91)90833-W
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
We describe a new model of submaximal stimulation of liver regeneration in the rat. Removal of the right lateral lobes in the rat produced a 21% hepatectomy. The measurement accuracy of the incorporation rate of [C-14]thymidine into DNA 24 h after 21% hepatectomy (n = 32) was less than that after the standard 34% hepatectomy (n = 32), with S.D./mean being 35% and 130%, respectively (F-test, p < 0.025). This model was able to detect regeneration-stimulation activity present in liver cytosol extract and serum of 68% hepatectomized rats. Using this model we identified a subfraction of rat serum achieved after treatment of serum with ethanol and ion-exchange column, which had a highly stimulatory effect. Stimulation obtained with serum from hepatectomized pigs showed that these factors are not species specific.
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页码:316 / 320
页数:5
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