ON THE CATALYTIC MECHANISM OF ECORI AND ECORV - A DETAILED PROPOSAL BASED ON BIOCHEMICAL RESULTS, STRUCTURAL DATA AND MOLECULAR MODELING

被引：67

作者：

JELTSCH, A ^{[1
]}

ALVES, J ^{[1
]}

MAASS, G ^{[1
]}

PINGOUD, A ^{[1
]}

机构：

[1] MED HSCH HANNOVER,ZENTRUM BIOCHEM,INST BIOPHYS CHEM,KONSTANTY GUTSCHOW STR 8,W-3000 HANNOVER 61,GERMANY

来源：

FEBS LETTERS | 1992年 / 304卷 / 01期

关键词：

ECORI; ECORV; DNA CLEAVAGE; CATALYTIC MECHANISM; MOLECULAR MODELING;

D O I：

10.1016/0014-5793(92)80576-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

EcoRI and EcoRV have a very similar active site, as is apparent from a comparison of the structures of their respective protein-DNA complexes. Based on structural and mechanistic data, as well as detailed molecular modelling presented here, a mechanism for the DNA cleavage by these enzymes is suggested in which the attacking water molecule is activated by the phosphate group 3' to the scissile phosphodiester bond, and in which the leaving group is protonated by a water molecule associated with the essential cofactor, Mg2+. The mechanism proposed may also apply to other nucleases.

引用

页码：4 / 8

页数：5

共 31 条

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