HIGH-RESOLUTION PROTON NMR INVESTIGATIONS OF RAT-BLOOD PLASMA - ASSIGNMENT OF RESONANCES FOR THE MOLECULARLY MOBILE CARBOHYDRATE SIDE-CHAINS OF ACUTE-PHASE GLYCOPROTEINS

被引：36

作者：

GROOTVELD, M ^{[1
]}

CLAXSON, AWD ^{[1
]}

CHANDER, CL ^{[1
]}

HAYCOCK, P ^{[1
]}

BLAKE, DR ^{[1
]}

HAWKES, GE ^{[1
]}

机构：

[1] QUEEN MARY & WESTFIELD COLL,DEPT CHEM,LONDON E1 4NS,ENGLAND

来源：

FEBS LETTERS | 1993年 / 322卷 / 03期

关键词：

H-1-NMR; RAT PLASMA; ACUTE-PHASE GLYCOPROTEIN; O-ACETYLSIALATE; N-ACETYLATED SUGAR; INFLAMMATION;

D O I：

10.1016/0014-5793(93)81584-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An intense broad resonance at 2.14 ppm present in high field (400, 500 and 600 MHz) Hahn spin-echo H-1-NMR spectra of rat blood plasma, but absent from those of human blood plasma is attributable to the presence of terminal 0-acetylsialate sugars in the molecularly mobile carbohydrate side-chains of 'acute-phase' glycoproteins (predominantly alpha1-acid glycoprotein). The presence of such alternative acetylsugars in the carbohydrate side-chains of rat plasma glycoproteins are of much physiological and experimental significance in view of the regular use of these animals in model systems of human inflammatory conditions.

引用

页码：266 / 276

页数：11

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