THE 5'-UNTRANSLATED REGION OF THE IGF-I RECEPTOR GENE MODULATES REPORTER GENE-EXPRESSION BY BOTH PRETRANSCRIPTIONAL AND POSTTRANSCRIPTIONAL MECHANISMS

被引：10

作者：

COOKE, DW ^{[1
]}

CASELLA, SJ ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,DEPT PEDIAT,BALTIMORE,MD 21218

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1994年 / 101卷 / 1-2期

关键词：

INSULIN-LIKE GROWTH FACTOR; RECEPTOR; INSULIN-LIKE GROWTH FACTOR RECEPTOR; 5'-UNTRANSLATED REGION; GENE EXPRESSION;

D O I：

10.1016/0303-7207(94)90221-6

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The insulin-like growth factor I(IGF-I) receptor gene has a large, complex 5'-untranslated region (UTR). In order to examine the role that this region plays in regulating IGF-I receptor expression, we isolated fragments of the human IGF-I receptor 5'-UTR and interposed them between the SV40 early promoter and the chloramphenicol acetyl transferase reporter gene. Fragments of the IGF-I receptor gene 5'-UTR were found to enhance reporter gene expression by increasing gene transcription. In addition, the increased transcription and mRNA levels were in excess of the increase in enzyme activity, providing indirect evidence that these fragments inhibit translation, consistent with predictions.

引用

页码：77 / 84

页数：8

共 48 条

[1]

ADASHI EY, 1986, J BIOL CHEM, V261, P3923

[2] INHIBITION OF TRANSLATION OF TRANSFORMING GROWTH FACTOR-BETA-3 MESSENGER-RNA BY ITS 5' UNTRANSLATED REGION [J].