EFFICIENT RECOVERY OF RECOMBINANT PROTEINS USING MEMBRANE-BASED IMMUNOAFFINITY CHROMATOGRAPHY (MIC)

被引:26
作者
NACHMAN, M [1 ]
AZAD, ARM [1 ]
BAILON, P [1 ]
机构
[1] SEPRACOR INC,MARLBOROUGH,MA 01752
关键词
MEMBRANES; IMMUNOAFFINITY; RECOMBINANT; INTERFERON; INTERLEUKIN-2; RECEPTOR;
D O I
10.1002/bit.260400503
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A systematic approach to the design and development of membrane-based immunoaffinity systems for the purification of recombinant proteins is presented. The preparation and characterization of immunoaffinity membranes are described. The immunoaffinity purification process for recombinant interferon-alpha-2a is used as a model system to determine the operational parameters in membrane-based immunoaffinity chromatography. The high volumetric throughput of membranes, combined with the typically fast-binding kinetics of antigen-antibody interactions, enable the purification of recombinant proteins from dilute feed stream in less time, using less antibody than conventional systems. Three recombinant proteins, human interferon-alpha-2a, interleukin-2, and interleukin-2 receptor, have been purified efficiently employing membrane-based immunoaffinity chromatography. Overall, membrane-based immunoaffinity chromatography is shown to be a viable and scalable method, ideal for the industrial-scale production of recombinant proteins.
引用
收藏
页码:564 / 571
页数:8
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