CHARACTERIZATION OF THE RHIZOBIUM-LEGUMINOSARUM BIOVAR PHASEOLI NIFA GENE, A POSITIVE REGULATOR OF NIF GENE-EXPRESSION

被引：29

作者：

MICHIELS, J ^{[1
]}

DHOOGHE, I ^{[1
]}

VERRETH, C ^{[1
]}

PELEMANS, H ^{[1
]}

VANDERLEYDEN, J ^{[1
]}

机构：

[1] CATHOLIC UNIV LEUVEN,FA JANSSENS LAB GENET,B-3001 HEVERLEE,BELGIUM

来源：

ARCHIVES OF MICROBIOLOGY | 1994年 / 161卷 / 05期

关键词：

RHIZOBIUM LEGUMINOSARUM BIOVAR PHASEOLI; NIFA; NIFB; NIFH; NITROGEN FIXATION; NIF GENE; FIX GENE;

D O I：

10.1007/s002030050073

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We report the isolation, mutational analysis and the nucleotide sequence of the Rhizobium leguminosarum bv. phaseoli nifA gene. Comparison of the deduced amino acid sequence with other NifA sequences indicated the presence of the conserved central activator and the C-terminal DNA-binding domains. Nodules elicited by a R. leguminosarum bv. phaseoli nifA mutant were symbiotically ineffective. The expression of a nifA-gusA fusion was shown to be independent on the oxygen status of the cell. We cloned the three nifH copies of R. leguminosarum bv. phaseoli and determined the nucleotide sequence of their promoter regions. The expression of nifH-gusA fusions is induced under microaerobic conditions and is dependent on the presence of NifA.

引用

页码：404 / 408

页数：5

共 25 条

[1] NUCLEOTIDE-SEQUENCE OF A 24,206-BASE-PAIR DNA FRAGMENT CARRYING THE ENTIRE NITROGEN-FIXATION GENE-CLUSTER OF KLEBSIELLA-PNEUMONIAE [J].

ARNOLD, W ;

RUMP, A ;

KLIPP, W ;

PRIEFER, UB ;

PUHLER, A .

JOURNAL OF MOLECULAR BIOLOGY, 1988, 203 (03) :715-738

[2] FIXK, A GENE HOMOLOGOUS WITH FNR AND CRP FROM ESCHERICHIA-COLI, REGULATES NITROGEN-FIXATION GENES BOTH POSITIVELY AND NEGATIVELY IN RHIZOBIUM-MELILOTI [J].

BATUT, J ;

DAVERANMINGOT, ML ;

JACOBS, MDJ ;

GARNERONE, AM ;

KAHN, D .

EMBO JOURNAL, 1989, 8 (04) :1279-1286

[3] EXPRESSION AND FUNCTIONAL-ANALYSIS OF THE RHIZOBIUM-MELILOTI-NIFA GENE [J].

BEYNON, JL ;

WILLIAMS, MK ;

CANNON, FC .

EMBO JOURNAL, 1988, 7 (01) :7-14

[4] IDENTIFICATION OF 2 CLASSES OF RHIZOBIUM-PHASEOLI GENES REQUIRED FOR MELANIN SYNTHESIS, ONE OF WHICH IS REQUIRED FOR NITROGEN-FIXATION AND ACTIVATES THE TRANSCRIPTION OF THE OTHER [J].

BORTHAKUR, D ;

LAMB, JW ;

JOHNSTON, AWB .

MOLECULAR & GENERAL GENETICS, 1987, 207 (01) :155-160

[5] ROLE OF THE NIFA GENE-PRODUCT IN THE REGULATION OF NIF EXPRESSION IN KLEBSIELLA-PNEUMONIAE [J].

BUCHANANWOLLASTON, V ;

CANNON, MC ;

BEYNON, JL ;

CANNON, FC .

NATURE, 1981, 294 (5843) :776-778

[6]

CHOONGHYUN K, 1986, GENE, V50, P141

[7] CASCADE REGULATION OF NIF GENE-EXPRESSION IN RHIZOBIUM-MELILOTI [J].

DAVID, M ;

DAVERAN, ML ;

BATUT, J ;

DEDIEU, A ;

DOMERGUE, O ;

GHAI, J ;

HERTIG, C ;

BOISTARD, P ;

KAHN, D .

CELL, 1988, 54 (05) :671-683

[8] ESSENTIAL AND NON-ESSENTIAL DOMAINS IN THE BRADYRHIZOBIUM-JAPONICUM NIFA PROTEIN - IDENTIFICATION OF INDISPENSABLE CYSTEINE RESIDUES POTENTIALLY INVOLVED IN REDOX REACTIVITY AND OR METAL-BINDING [J].

FISCHER, HM ;

BRUDERER, T ;

HENNECKE, H .

NUCLEIC ACIDS RESEARCH, 1988, 16 (05) :2207-2224

[9] REGULATION OF NITROGEN-FIXATION GENES [J].

GUSSIN, GN ;

RONSON, CW ;

AUSUBEL, FM .

ANNUAL REVIEW OF GENETICS, 1986, 20 :567-591

[10] A FAMILY OF RELATED ATP-BINDING SUBUNITS COUPLED TO MANY DISTINCT BIOLOGICAL PROCESSES IN BACTERIA [J].

HIGGINS, CF ;

HILES, ID ;

SALMOND, GPC ;

GILL, DR ;

DOWNIE, JA ;

EVANS, IJ ;

HOLLAND, IB ;

GRAY, L ;

BUCKEL, SD ;

BELL, AW ;

HERMODSON, MA .

NATURE, 1986, 323 (6087) :448-450

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