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DEVELOPMENT OF A SIMPLE PARTICLE BOMBARDMENT DEVICE FOR GENE-TRANSFER INTO PLANT-CELLS

被引：27
作者：
BROWN, DCW
TIAN, LN
BUCKLEY, DJ
LEFEBVRE, M
MCGRATH, A
WEBB, J
机构：
[1] AGR CANADA,CTR FOOD & ANIM RES,OTTAWA,ON,CANADA
[2] CARLETON UNIV,DEPT BIOCHEM,OTTAWA,ON,CANADA
[3] UNIV GUELPH,DEPT CROP SCI,GUELPH N1G 2W1,ON,CANADA
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 1994年 / 37卷 / 01期
关键词：
TRANSIENT GENE EXPRESSION; BETA-GLUCURONIDASE; ALFALFA; SOYBEAN; DNA TRANSFER;
D O I：
10.1007/BF00048116
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A simple particle bombardment device was designed, constructed and shown to be efficient for the delivery of DNA into plant cells. High levels of transient beta-glucuronidase expression were observed in alfalfa suspension-cultured cells and embryogenic soybean suspension-cultured cells. Expression of beta-glucuronidase in alfalfa suspension-cultured cells was used to optimize the bombardment conditions for the device. Transient gene expression in alfalfa was found to be dependent on the state of the target tissue, the size of particles employed, the helium pressure used to accelerate the particles and the distance travel led by the tungsten particles carrying DNA.
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页码：47 / 53
页数：7
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