PURIFICATION AND SOME PHYSICOCHEMICAL PROPERTIES OF STAPHYLOCOCCAL ENTEROTOXIN-D

被引:33
作者
CHANG, HC [1 ]
BERGDOLL, MS [1 ]
机构
[1] UNIV WISCONSIN, INST FOOD RES, MADISON, WI 53706 USA
关键词
D O I
10.1021/bi00577a013
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A method was developed for the isolation of staphylococcal enterotoxin D in highly purified form from cultures of Staphylococcus aureus strain 1151m. The method involves removal of the toxin from the culture supernatant fluid with the ion-exchange resin CG-50 followed by chromatography on carboxymethylcellulose (twice) and by gel filtration on Sephadex G-75 (twice). The purified toxin is homogeneous by polyacrylamide gel and sodium dodecyl sulfate-poly-acrylamide gel electrophoresis and double gel diffusion tests. It is a simple, colorless, antigenic protein with an isoelectric point of 7.4 as determined by isoelectric focusing. Its molecular weight was determined to be 27 300 ± 700 by molecular sieve chromatography on Sephadex G-100 and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Its serological activity is stable over a wide range of pH values (1.2-10.7). The enterotoxin consists of 236 amino acid residues and contains no free sulfhydryl groups. End-group analysis showed serine to be the NH2-terminal amino acid and lysine to be the COOH-terminal amino acid. © 1979, American Chemical Society. All rights reserved.
引用
收藏
页码:1937 / 1942
页数:6
相关论文
共 42 条