FUNCTION OF THE CONSERVED TRIAD RESIDUES IN THE CLASS-C BETA-LACTAMASE FROM CITROBACTER-FREUNDII GN346

被引：27

作者：

TSUKAMOTO, K ^{[1
]}

NISHIDA, N ^{[1
]}

TSURUOKA, M ^{[1
]}

SAWAI, T ^{[1
]}

机构：

[1] CHIBA UNIV,FAC PHARMACEUT SCI,DIV MICROBIAL CHEM,1-33 YAYOICHO,CHIBA 260,JAPAN

来源：

FEBS LETTERS | 1990年 / 271卷 / 1-2期

关键词：

Active site; Cephalosporinase; Citrobacter freundii; Conserved residue; Site-directed mutagenesis; β-Lactamase;

D O I：

10.1016/0014-5793(90)80416-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The conserved KTG triad in the class C β-lactamase from Citrobacter freundii GN346 was examined as to its function by means of site-directed mutagenesis. The following conversions were performed; Lys-315 to arginine, alanine or glutamic acid, Thr-316 to valine, and Gly-317 to alanine, proline or isoleucine. The resultant mutant enzymes revealed that a basic amino acid at position 315 and a small uncharged residue at position 317 are essential for the enzyme activity, but a hydroxyl group at residue 316 is not required for the enzymatic catalysis. The kinetic properties of the purified Arg-315 and Val-316 enzymes provided information on the function of these residues. © 1990.

引用

页码：243 / 246

页数：4

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