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FUNCTION OF THE CONSERVED TRIAD RESIDUES IN THE CLASS-C BETA-LACTAMASE FROM CITROBACTER-FREUNDII GN346
被引:27
作者
:
TSUKAMOTO, K
论文数:
0
引用数:
0
h-index:
0
机构:
CHIBA UNIV,FAC PHARMACEUT SCI,DIV MICROBIAL CHEM,1-33 YAYOICHO,CHIBA 260,JAPAN
CHIBA UNIV,FAC PHARMACEUT SCI,DIV MICROBIAL CHEM,1-33 YAYOICHO,CHIBA 260,JAPAN
TSUKAMOTO, K
[
1
]
NISHIDA, N
论文数:
0
引用数:
0
h-index:
0
机构:
CHIBA UNIV,FAC PHARMACEUT SCI,DIV MICROBIAL CHEM,1-33 YAYOICHO,CHIBA 260,JAPAN
CHIBA UNIV,FAC PHARMACEUT SCI,DIV MICROBIAL CHEM,1-33 YAYOICHO,CHIBA 260,JAPAN
NISHIDA, N
[
1
]
TSURUOKA, M
论文数:
0
引用数:
0
h-index:
0
机构:
CHIBA UNIV,FAC PHARMACEUT SCI,DIV MICROBIAL CHEM,1-33 YAYOICHO,CHIBA 260,JAPAN
CHIBA UNIV,FAC PHARMACEUT SCI,DIV MICROBIAL CHEM,1-33 YAYOICHO,CHIBA 260,JAPAN
TSURUOKA, M
[
1
]
SAWAI, T
论文数:
0
引用数:
0
h-index:
0
机构:
CHIBA UNIV,FAC PHARMACEUT SCI,DIV MICROBIAL CHEM,1-33 YAYOICHO,CHIBA 260,JAPAN
CHIBA UNIV,FAC PHARMACEUT SCI,DIV MICROBIAL CHEM,1-33 YAYOICHO,CHIBA 260,JAPAN
SAWAI, T
[
1
]
机构
:
[1]
CHIBA UNIV,FAC PHARMACEUT SCI,DIV MICROBIAL CHEM,1-33 YAYOICHO,CHIBA 260,JAPAN
来源
:
FEBS LETTERS
|
1990年
/ 271卷
/ 1-2期
关键词
:
Active site;
Cephalosporinase;
Citrobacter freundii;
Conserved residue;
Site-directed mutagenesis;
β-Lactamase;
D O I
:
10.1016/0014-5793(90)80416-G
中图分类号
:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号
:
071010 ;
081704 ;
摘要
:
The conserved KTG triad in the class C β-lactamase from Citrobacter freundii GN346 was examined as to its function by means of site-directed mutagenesis. The following conversions were performed; Lys-315 to arginine, alanine or glutamic acid, Thr-316 to valine, and Gly-317 to alanine, proline or isoleucine. The resultant mutant enzymes revealed that a basic amino acid at position 315 and a small uncharged residue at position 317 are essential for the enzyme activity, but a hydroxyl group at residue 316 is not required for the enzymatic catalysis. The kinetic properties of the purified Arg-315 and Val-316 enzymes provided information on the function of these residues. © 1990.
引用
收藏
页码:243 / 246
页数:4
相关论文
共 15 条
[1]
THE STRUCTURE OF BETA-LACTAMASES
AMBLER, RP
论文数:
0
引用数:
0
h-index:
0
AMBLER, RP
[J].
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES,
1980,
289
(1036)
: 321
-
331
[2]
THE ACTIVE-SITE-SERINE PENICILLIN-RECOGNIZING ENZYMES AS MEMBERS OF THE STREPTOMYCES R61 DD-PEPTIDASE FAMILY
JORIS, B
论文数:
0
引用数:
0
h-index:
0
机构:
EUROGENTEC SA, B-4000 LIEGE, BELGIUM
JORIS, B
GHUYSEN, JM
论文数:
0
引用数:
0
h-index:
0
机构:
EUROGENTEC SA, B-4000 LIEGE, BELGIUM
GHUYSEN, JM
DIVE, G
论文数:
0
引用数:
0
h-index:
0
机构:
EUROGENTEC SA, B-4000 LIEGE, BELGIUM
DIVE, G
RENARD, A
论文数:
0
引用数:
0
h-index:
0
机构:
EUROGENTEC SA, B-4000 LIEGE, BELGIUM
RENARD, A
DIDEBERG, O
论文数:
0
引用数:
0
h-index:
0
机构:
EUROGENTEC SA, B-4000 LIEGE, BELGIUM
DIDEBERG, O
CHARLIER, P
论文数:
0
引用数:
0
h-index:
0
机构:
EUROGENTEC SA, B-4000 LIEGE, BELGIUM
CHARLIER, P
FRERE, JM
论文数:
0
引用数:
0
h-index:
0
机构:
EUROGENTEC SA, B-4000 LIEGE, BELGIUM
FRERE, JM
KELLY, JA
论文数:
0
引用数:
0
h-index:
0
机构:
EUROGENTEC SA, B-4000 LIEGE, BELGIUM
KELLY, JA
BOYINGTON, JC
论文数:
0
引用数:
0
h-index:
0
机构:
EUROGENTEC SA, B-4000 LIEGE, BELGIUM
BOYINGTON, JC
MOEWS, PC
论文数:
0
引用数:
0
h-index:
0
机构:
EUROGENTEC SA, B-4000 LIEGE, BELGIUM
MOEWS, PC
KNOX, JR
论文数:
0
引用数:
0
h-index:
0
机构:
EUROGENTEC SA, B-4000 LIEGE, BELGIUM
KNOX, JR
[J].
BIOCHEMICAL JOURNAL,
1988,
250
(02)
: 313
-
324
[3]
Miller J.H., 1972, EXPT MOL GENETICS, P1
[4]
MICRO-IODOMETRIC ASSAY FOR PENICILLINASE
NOVICK, RP
论文数:
0
引用数:
0
h-index:
0
NOVICK, RP
[J].
BIOCHEMICAL JOURNAL,
1962,
83
(02)
: 236
-
&
[5]
REFINED CRYSTAL-STRUCTURE OF BETA-LACTAMASE FROM CITROBACTER-FREUNDII INDICATES A MECHANISM FOR BETA-LACTAM HYDROLYSIS
OEFNER, C
论文数:
0
引用数:
0
h-index:
0
机构:
F HOFFMANN LA ROCHE & CO LTD, CENT RES UNIT, CH-4002 BASEL, SWITZERLAND
OEFNER, C
DARCY, A
论文数:
0
引用数:
0
h-index:
0
机构:
F HOFFMANN LA ROCHE & CO LTD, CENT RES UNIT, CH-4002 BASEL, SWITZERLAND
DARCY, A
DALY, JJ
论文数:
0
引用数:
0
h-index:
0
机构:
F HOFFMANN LA ROCHE & CO LTD, CENT RES UNIT, CH-4002 BASEL, SWITZERLAND
DALY, JJ
GUBERNATOR, K
论文数:
0
引用数:
0
h-index:
0
机构:
F HOFFMANN LA ROCHE & CO LTD, CENT RES UNIT, CH-4002 BASEL, SWITZERLAND
GUBERNATOR, K
CHARNAS, RL
论文数:
0
引用数:
0
h-index:
0
机构:
F HOFFMANN LA ROCHE & CO LTD, CENT RES UNIT, CH-4002 BASEL, SWITZERLAND
CHARNAS, RL
HEINZE, I
论文数:
0
引用数:
0
h-index:
0
机构:
F HOFFMANN LA ROCHE & CO LTD, CENT RES UNIT, CH-4002 BASEL, SWITZERLAND
HEINZE, I
HUBSCHWERLEN, C
论文数:
0
引用数:
0
h-index:
0
机构:
F HOFFMANN LA ROCHE & CO LTD, CENT RES UNIT, CH-4002 BASEL, SWITZERLAND
HUBSCHWERLEN, C
WINKLER, FK
论文数:
0
引用数:
0
h-index:
0
机构:
F HOFFMANN LA ROCHE & CO LTD, CENT RES UNIT, CH-4002 BASEL, SWITZERLAND
WINKLER, FK
[J].
NATURE,
1990,
343
(6255)
: 284
-
288
[6]
DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS
SANGER, F
论文数:
0
引用数:
0
h-index:
0
SANGER, F
NICKLEN, S
论文数:
0
引用数:
0
h-index:
0
NICKLEN, S
COULSON, AR
论文数:
0
引用数:
0
h-index:
0
COULSON, AR
[J].
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,
1977,
74
(12)
: 5463
-
5467
[7]
SAWAI T, 1988, REV INFECT DIS, V10, P721
[8]
A SET OF BACTERIAL STRAINS FOR EVALUATION OF BETA-LACTAMASE-STABILITY OF BETA-LACTAM ANTIBIOTICS
SAWAI, T
论文数:
0
引用数:
0
h-index:
0
SAWAI, T
YOSHIDA, T
论文数:
0
引用数:
0
h-index:
0
YOSHIDA, T
TSUKAMOTO, K
论文数:
0
引用数:
0
h-index:
0
TSUKAMOTO, K
YAMAGISHI, S
论文数:
0
引用数:
0
h-index:
0
YAMAGISHI, S
[J].
JOURNAL OF ANTIBIOTICS,
1981,
34
(10)
: 1318
-
1326
[9]
SAWAI T, 1982, J BACTERIOL, V152, P567
[10]
THE USE OF PHOSPHOROTHIOATE-MODIFIED DNA IN RESTRICTION ENZYME-REACTIONS TO PREPARE NICKED DNA
TAYLOR, JW
论文数:
0
引用数:
0
h-index:
0
机构:
MAX PLANCK INST EXPTL MED, CHEM ABT, D-3400 GOTTINGEN, GERMANY
MAX PLANCK INST EXPTL MED, CHEM ABT, D-3400 GOTTINGEN, GERMANY
TAYLOR, JW
SCHMIDT, W
论文数:
0
引用数:
0
h-index:
0
机构:
MAX PLANCK INST EXPTL MED, CHEM ABT, D-3400 GOTTINGEN, GERMANY
MAX PLANCK INST EXPTL MED, CHEM ABT, D-3400 GOTTINGEN, GERMANY
SCHMIDT, W
COSSTICK, R
论文数:
0
引用数:
0
h-index:
0
机构:
MAX PLANCK INST EXPTL MED, CHEM ABT, D-3400 GOTTINGEN, GERMANY
MAX PLANCK INST EXPTL MED, CHEM ABT, D-3400 GOTTINGEN, GERMANY
COSSTICK, R
OKRUSZEK, A
论文数:
0
引用数:
0
h-index:
0
机构:
MAX PLANCK INST EXPTL MED, CHEM ABT, D-3400 GOTTINGEN, GERMANY
MAX PLANCK INST EXPTL MED, CHEM ABT, D-3400 GOTTINGEN, GERMANY
OKRUSZEK, A
ECKSTEIN, F
论文数:
0
引用数:
0
h-index:
0
机构:
MAX PLANCK INST EXPTL MED, CHEM ABT, D-3400 GOTTINGEN, GERMANY
MAX PLANCK INST EXPTL MED, CHEM ABT, D-3400 GOTTINGEN, GERMANY
ECKSTEIN, F
[J].
NUCLEIC ACIDS RESEARCH,
1985,
13
(24)
: 8749
-
8764
←
1
2
→
共 15 条
[1]
THE STRUCTURE OF BETA-LACTAMASES
AMBLER, RP
论文数:
0
引用数:
0
h-index:
0
AMBLER, RP
[J].
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES,
1980,
289
(1036)
: 321
-
331
[2]
THE ACTIVE-SITE-SERINE PENICILLIN-RECOGNIZING ENZYMES AS MEMBERS OF THE STREPTOMYCES R61 DD-PEPTIDASE FAMILY
JORIS, B
论文数:
0
引用数:
0
h-index:
0
机构:
EUROGENTEC SA, B-4000 LIEGE, BELGIUM
JORIS, B
GHUYSEN, JM
论文数:
0
引用数:
0
h-index:
0
机构:
EUROGENTEC SA, B-4000 LIEGE, BELGIUM
GHUYSEN, JM
DIVE, G
论文数:
0
引用数:
0
h-index:
0
机构:
EUROGENTEC SA, B-4000 LIEGE, BELGIUM
DIVE, G
RENARD, A
论文数:
0
引用数:
0
h-index:
0
机构:
EUROGENTEC SA, B-4000 LIEGE, BELGIUM
RENARD, A
DIDEBERG, O
论文数:
0
引用数:
0
h-index:
0
机构:
EUROGENTEC SA, B-4000 LIEGE, BELGIUM
DIDEBERG, O
CHARLIER, P
论文数:
0
引用数:
0
h-index:
0
机构:
EUROGENTEC SA, B-4000 LIEGE, BELGIUM
CHARLIER, P
FRERE, JM
论文数:
0
引用数:
0
h-index:
0
机构:
EUROGENTEC SA, B-4000 LIEGE, BELGIUM
FRERE, JM
KELLY, JA
论文数:
0
引用数:
0
h-index:
0
机构:
EUROGENTEC SA, B-4000 LIEGE, BELGIUM
KELLY, JA
BOYINGTON, JC
论文数:
0
引用数:
0
h-index:
0
机构:
EUROGENTEC SA, B-4000 LIEGE, BELGIUM
BOYINGTON, JC
MOEWS, PC
论文数:
0
引用数:
0
h-index:
0
机构:
EUROGENTEC SA, B-4000 LIEGE, BELGIUM
MOEWS, PC
KNOX, JR
论文数:
0
引用数:
0
h-index:
0
机构:
EUROGENTEC SA, B-4000 LIEGE, BELGIUM
KNOX, JR
[J].
BIOCHEMICAL JOURNAL,
1988,
250
(02)
: 313
-
324
[3]
Miller J.H., 1972, EXPT MOL GENETICS, P1
[4]
MICRO-IODOMETRIC ASSAY FOR PENICILLINASE
NOVICK, RP
论文数:
0
引用数:
0
h-index:
0
NOVICK, RP
[J].
BIOCHEMICAL JOURNAL,
1962,
83
(02)
: 236
-
&
[5]
REFINED CRYSTAL-STRUCTURE OF BETA-LACTAMASE FROM CITROBACTER-FREUNDII INDICATES A MECHANISM FOR BETA-LACTAM HYDROLYSIS
OEFNER, C
论文数:
0
引用数:
0
h-index:
0
机构:
F HOFFMANN LA ROCHE & CO LTD, CENT RES UNIT, CH-4002 BASEL, SWITZERLAND
OEFNER, C
DARCY, A
论文数:
0
引用数:
0
h-index:
0
机构:
F HOFFMANN LA ROCHE & CO LTD, CENT RES UNIT, CH-4002 BASEL, SWITZERLAND
DARCY, A
DALY, JJ
论文数:
0
引用数:
0
h-index:
0
机构:
F HOFFMANN LA ROCHE & CO LTD, CENT RES UNIT, CH-4002 BASEL, SWITZERLAND
DALY, JJ
GUBERNATOR, K
论文数:
0
引用数:
0
h-index:
0
机构:
F HOFFMANN LA ROCHE & CO LTD, CENT RES UNIT, CH-4002 BASEL, SWITZERLAND
GUBERNATOR, K
CHARNAS, RL
论文数:
0
引用数:
0
h-index:
0
机构:
F HOFFMANN LA ROCHE & CO LTD, CENT RES UNIT, CH-4002 BASEL, SWITZERLAND
CHARNAS, RL
HEINZE, I
论文数:
0
引用数:
0
h-index:
0
机构:
F HOFFMANN LA ROCHE & CO LTD, CENT RES UNIT, CH-4002 BASEL, SWITZERLAND
HEINZE, I
HUBSCHWERLEN, C
论文数:
0
引用数:
0
h-index:
0
机构:
F HOFFMANN LA ROCHE & CO LTD, CENT RES UNIT, CH-4002 BASEL, SWITZERLAND
HUBSCHWERLEN, C
WINKLER, FK
论文数:
0
引用数:
0
h-index:
0
机构:
F HOFFMANN LA ROCHE & CO LTD, CENT RES UNIT, CH-4002 BASEL, SWITZERLAND
WINKLER, FK
[J].
NATURE,
1990,
343
(6255)
: 284
-
288
[6]
DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS
SANGER, F
论文数:
0
引用数:
0
h-index:
0
SANGER, F
NICKLEN, S
论文数:
0
引用数:
0
h-index:
0
NICKLEN, S
COULSON, AR
论文数:
0
引用数:
0
h-index:
0
COULSON, AR
[J].
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,
1977,
74
(12)
: 5463
-
5467
[7]
SAWAI T, 1988, REV INFECT DIS, V10, P721
[8]
A SET OF BACTERIAL STRAINS FOR EVALUATION OF BETA-LACTAMASE-STABILITY OF BETA-LACTAM ANTIBIOTICS
SAWAI, T
论文数:
0
引用数:
0
h-index:
0
SAWAI, T
YOSHIDA, T
论文数:
0
引用数:
0
h-index:
0
YOSHIDA, T
TSUKAMOTO, K
论文数:
0
引用数:
0
h-index:
0
TSUKAMOTO, K
YAMAGISHI, S
论文数:
0
引用数:
0
h-index:
0
YAMAGISHI, S
[J].
JOURNAL OF ANTIBIOTICS,
1981,
34
(10)
: 1318
-
1326
[9]
SAWAI T, 1982, J BACTERIOL, V152, P567
[10]
THE USE OF PHOSPHOROTHIOATE-MODIFIED DNA IN RESTRICTION ENZYME-REACTIONS TO PREPARE NICKED DNA
TAYLOR, JW
论文数:
0
引用数:
0
h-index:
0
机构:
MAX PLANCK INST EXPTL MED, CHEM ABT, D-3400 GOTTINGEN, GERMANY
MAX PLANCK INST EXPTL MED, CHEM ABT, D-3400 GOTTINGEN, GERMANY
TAYLOR, JW
SCHMIDT, W
论文数:
0
引用数:
0
h-index:
0
机构:
MAX PLANCK INST EXPTL MED, CHEM ABT, D-3400 GOTTINGEN, GERMANY
MAX PLANCK INST EXPTL MED, CHEM ABT, D-3400 GOTTINGEN, GERMANY
SCHMIDT, W
COSSTICK, R
论文数:
0
引用数:
0
h-index:
0
机构:
MAX PLANCK INST EXPTL MED, CHEM ABT, D-3400 GOTTINGEN, GERMANY
MAX PLANCK INST EXPTL MED, CHEM ABT, D-3400 GOTTINGEN, GERMANY
COSSTICK, R
OKRUSZEK, A
论文数:
0
引用数:
0
h-index:
0
机构:
MAX PLANCK INST EXPTL MED, CHEM ABT, D-3400 GOTTINGEN, GERMANY
MAX PLANCK INST EXPTL MED, CHEM ABT, D-3400 GOTTINGEN, GERMANY
OKRUSZEK, A
ECKSTEIN, F
论文数:
0
引用数:
0
h-index:
0
机构:
MAX PLANCK INST EXPTL MED, CHEM ABT, D-3400 GOTTINGEN, GERMANY
MAX PLANCK INST EXPTL MED, CHEM ABT, D-3400 GOTTINGEN, GERMANY
ECKSTEIN, F
[J].
NUCLEIC ACIDS RESEARCH,
1985,
13
(24)
: 8749
-
8764
←
1
2
→