A COMPETITIVE ENZYME-IMMUNOASSAY FOR ALBUTEROL - ITS APPLICATION FOR THE DRUG SCREENING IN URINE

被引：6

作者：

ADAM, A

ONG, H

GRAVEL, A

MESSIER, J

BELLEMARE, M

LANTIN, F

SAUVE, G

TYSSEN, P

机构：

[1] AGR CANADA,DIV HYG VIANDE & PROD VOLAILLE,OTTAWA,ONTARIO,CANADA

[2] BUR MED VET,SANTE & BIENETRE SOCIAL CANADA,OTTAWA K1A 1B7,ONTARIO,CANADA

[3] UNIV QUEBEC,INST ARMAND FRAPPIER,LAVAL H7N 4Z3,QUEBEC,CANADA

来源：

JOURNAL OF IMMUNOASSAY | 1991年 / 12卷 / 02期

关键词：

ALBUTEROL; CLENBUTEROL; ENZYME IMMUNOASSAY; MEAT PRODUCTION; DRUG RESIDUES;

D O I：

10.1080/01971529108055067

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A competitive enzyme immunoassay using purified monoclonal IgGl and an alkaline phosphatase-albuterol derivative has been developed for the quantification of albuterol in urine. The calibration curve obtained in optimal incubation conditions is characterized by a minimum detectable level of 26 fmol/well and a working range from 52 fmol to 4,2 pmol/well. This method allows the precise and accurate quantification of albuterol in horse urine without any clean up or extraction step. Moreover the definition of its specificity shows a cross-reactivity of the antibody with the glucurono-/sulfo-conjugates of albuterol. This property is particularly interesting for the screening of urinary albuterol residues in meat producing animals.

引用

页码：207 / 223

页数：17

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