SPORADIC CUTANEOUS LEISHMANIASIS IN NORTH TUNISIA - IDENTIFICATION OF THE CAUSATIVE AGENT AS LEISHMANIA-INFANTUM BY THE USE OF A DIAGNOSTIC DEOXYRIBONUCLEIC-ACID PROBE

被引：35

作者：

BENISMAIL, R

SMITH, DF

READY, PD

AYADI, A

GRAMICCIA, M

BENOSMAN, A

BENRACHID, MS

机构：

[1] FAC MED TUNIS,DEPT PARASITOL,TUNIS,TUNISIA

[2] UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED,DEPT BIOCHEM,LONDON SW7 2AZ,ENGLAND

[3] NAT HIST MUSEUM,DEPT ENTOMOL,LONDON,ENGLAND

[4] IST SUPER SANITA,PARASSITOL LAB,I-00161 ROME,ITALY

[5] SERV DERMATOL,TUNIS,TUNISIA

来源：

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE | 1992年 / 86卷 / 05期

关键词：

D O I：

10.1016/0035-9203(92)90087-S

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

A diagnostic kinetoplast deoxyribonucleic acid (DNA) probe has been used to identify as Leishmania infantum amastigotes taken from the lesions (in dermal fluid or lesion triturates) of 7 of 8 patients suffering from sporadic cutaneous leishmaniasis in north Tunisia. All 8 isolates were very difficult to culture but, subsequently, sufficient parasites of one isolate have been grown for isoenzyme typing, and were identified as zymodeme MON 24 of L. infantum. The advantages of using recombinant DNA probes for the identification of parasites from lesions are discussed.

引用

页码：508 / 510

页数：3

共 15 条

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[2]

BELAZZOUG S, 1985, ANN PARASIT HUM COMP, V60, P1

[3]

Ben Ismail R., 1989, Maladies tropicales transmissibles., P73

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