INFLUENCE OF EPTC (S-ETHYL-DIPROPYL-THIOCARBAMATE) ON THE COMPOSITION OF SURFACE WAXES AND SPOROPOLLENIN STRUCTURE IN ZEA-MAYS

被引：30

作者：

WILMESMEIER, S

WIERMANN, R

机构：

[1] Institut für Botanik, Westfälischen Wilhelms-Universität Münster, Münster, D-48149

来源：

JOURNAL OF PLANT PHYSIOLOGY | 1995年 / 146卷 / 1-2期

关键词：

ZEA MAYS; EPTC; FTIR SPECTROSCOPY; POLLEN; SPOROPOLLENIN; SURFACE WAVES;

D O I：

10.1016/S0176-1617(11)81962-0

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Thiocarbamate herbicides reduce the synthesis of very long chain fatty acids with C > 18. It was the aim of this study to reveal the involvement of lipid metabolism and especially of the chain-elongating enzyme systems in sporopollenin biosynthesis by treatment of Zea mays plants with the thiocarbamate EPTC. The distribution patterns of lipid classes from external pollen and leaf waxes were comparatively analysed from treated plants and controls. On this basis it was elucidated that the inhibitor is translocated in the plants, modifies the composition of lipid classes causing a shift to shorter chain lengths, and reaches the sites of sporopollenin biosynthesis, these being the anther loculus and the pollen. FTIR spectroscopic analyses of isolated and purified exines revealed significant changes in the band pattern of sporopollenin from treated plants, thus demonstrating the influence of EPTC on the chemical composition of sporopollenin by interaction with chain-elongating enzyme systems. The deviations from control spectra caused by EPTC are interpreted as an enhanced degree of polymer cross-linking by ether and/or ester bonds in order to compensate the reduced chain lengths of the aliphatic compounds that participate in the sporopollenin structure. This biochemical approach indicates that lipid metabolism and chain-elongating enzyme systems are involved in the synthesis of this remarkable biopolymer.

引用

页码：22 / 28

页数：7

共 36 条

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