RNA FROM INSECT TRYPANOSOME CRITHIDIA-LUCILIAE CONTAINS TRANSCRIPTS OF MAXI-CIRCLE AND NOT OF MINI-CIRCLE COMPONENT OF KINETOPLAST DNA

We have hybridized total cellular RNA of Crithidia luciliae with the kinetoplast DNA of this organism. To allow the discrimination of DNA from mini-circles (2300 base pairs) and maxi-circles (33 000 base pairs), kinetoplast DNA was digested with restriction endonucleases and the fragments were separated by electrophoresis through an agarose gel and transferred to nitrocellulose filters by blotting. No mini-circle transcripts were found under conditions where maxi-circle fragments showed extensive and specific hybridization. Since maxi-circle sequences are present at less than 1% of the concentration of mini-circle sequences, we conclude that mini-circles may not be transcribed at all. Predominant hybridization with the maxi-circle fragments is obtained with a segment of only 2300-2500 base pairs. The possibility that this segment codes for unusually small mitochondrial ribosomal RNAs is discussed. © 1978.