SEQUENCE COMPLEMENTARITY OF SONCHUS YELLOW NET VIRUS-RNA WITH RNA ISOLATED FROM THE POLYSOMES OF INFECTED TOBACCO

被引:20
作者
MILNER, JJ [1 ]
JACKSON, AO [1 ]
机构
[1] PURDUE UNIV, DEPT BOT & PLANT PATHOL, W LAFAYETTE, IN 47907 USA
基金
美国国家科学基金会;
关键词
D O I
10.1016/0042-6822(79)90375-1
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Polyribosomal RNA from tobacco infected with sonchus yellow net virus (SYNV) contained sequences which hybridized to 125I-labeled SYNV RNA and which were complementary to 80 to 100% of the viral RNA genome. The poly(A)-containing RNA from polyribosomes was complementary to over 90% of the viral genome but the polyribosomal RNA lacking poly(A) hybridized to approximately 40-60% of the genome. The kinetics of hybridization of all three fractions are best explained by the presence of a single abundance class of viral-complementary RNA. However, titration hybridization of poly(A)+ RNA to an excess of SYNV RNA suggested that viral-complementary sequences which contain poly(A) may vary in concentration over a factor of about fivefold. About 1.5 to 4.6% of the fraction containing poly(A), 0.02 to 0.06% of the fraction lacking poly(A) and 0.04 to 0.18% of the total polyribosomal RNA was complementary to viral RNA as estimated from the kinetics of hybridization. The viral complementary RNA(vcRNA) was heterogeneous in size with a modal sedimentation coefficient of 12 S and a profile in sucrose density gradients similar to the polyadenylated polyribosomal RNA. © 1979.
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页码:90 / 99
页数:10
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