QUANTITATIVE STUDY OF PROTEIN ASSOCIATION AT PICOMOLAR CONCENTRATIONS - THE LAMBDA PHAGE CL REPRESSOR

被引：35

作者：

BECKETT, D ^{[1
]}

KOBLAN, KS ^{[1
]}

ACKERS, GK ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,DEPT BIOL,BALTIMORE,MD 21218

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 196卷 / 01期

关键词：

D O I：

10.1016/0003-2697(91)90118-D

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method has been developed for radiolabeling the λ cl repressor to a specific activity sufficiently high to permit accurate quantitation of the protein in the picomolar range of concentration. Procedures are described whereby the labeled protein can be used for accurate quantitative study of the energetics of repressor assembly by large zone analytical gel chromatography. This methodology is applicable to other systems in which the stoichiometry and energetics of tightly associating DNA binding proteins are currently difficult to measure. © 1991.

引用

页码：69 / 75

页数：7

共 22 条

[1] Ackers G. K., 1975, PROTEINS, V1, P1
[2] AMMAN E, 1983, GENE, V25, P167
[3] INTERACTION OF THE ESCHERICHIA-COLI GAL REPRESSOR PROTEIN WITH ITS DNA OPERATORS INVITRO
BRENOWITZ, M
JAMISON, E
MAJUMDAR, A
ADHYA, S
[J]. BIOCHEMISTRY, 1990, 29 (13) : 3374 - 3383
[4] FOOTPRINT TITRATIONS YIELD VALID THERMODYNAMIC ISOTHERMS
BRENOWITZ, M
SENEAR, DF
SHEA, MA
ACKERS, GK
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (22) : 8462 - 8466
[5] BRENOWITZ M, 1986, METHOD ENZYMOL, V130, P132
[6] MECHANISM OF ACTION OF THE LEXA GENE-PRODUCT
BRENT, R
PTASHNE, M
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (07): : 4204 - 4208
[7] LAMBDA-PHAGE AND 434-PHAGE REPRESSORS
CHADWICK, P
PIRROTTA, V
STEINBER.R
HOPKINS, N
PTASHNE, M
[J]. COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1970, 35 : 283 - +
[8] MEASUREMENT OF DNA-PROTEIN EQUILIBRIA USING GEL CHROMATOGRAPHY - APPLICATION TO THE HINFI RESTRICTION ENDONUCLEASE
FRANKEL, AD
ACKERS, GK
SMITH, HO
[J]. BIOCHEMISTRY, 1985, 24 (12) : 3049 - 3054
[9] FRIED M, 1981, NUCLEIC ACIDS RES, V9, P6506
[10] A GEL-ELECTROPHORESIS METHOD FOR QUANTIFYING THE BINDING OF PROTEINS TO SPECIFIC DNA REGIONS - APPLICATION TO COMPONENTS OF THE ESCHERICHIA-COLI LACTOSE OPERON REGULATORY SYSTEM
GARNER, MM
REVZIN, A
[J]. NUCLEIC ACIDS RESEARCH, 1981, 9 (13) : 3047 - 3060

← 1 2 3 →