PREPARATION OF PROTOPLASTS AND WHOLE CELL GHOSTS FROM MYCOBACTERIUM-SMEGMATIS

被引：15

作者：

RASTOGI, N ^{[1
]}

VENKITASUBRAMANIAN, TA ^{[1
]}

机构：

[1] UNIV DELHI,VP CHEST INST,DEPT BIOCHEM,DELHI 110007,INDIA

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1979年 / 115卷 / DEC期

关键词：

D O I：

10.1099/00221287-115-2-517

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Cell wall-deficient forms of Mycobacterium smegmatis were produced in growth medium containing D-cycloserine and horse serum. These cells were transformed into protoplasts with EDTA and lysozyme. Subsequent lysis by nucleases followed by osmotic shock produced membrane vesicles (whole cell ghosts).

引用

页码：517 / 521

页数：5

共 18 条

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