MODIFIED METHOD OF MAMMALIAN-CELL SYNCHRONIZATION IMPROVES YIELD AND DEGREE OF SYNCHRONIZATION

被引：16

作者：

CAO, G ^{[1
]}

LIU, LM ^{[1
]}

CLEARY, SF ^{[1
]}

机构：

[1] VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT PHYSIOL & BIOPHYS,BOX 551,MCV STN,RICHMOND,VA 23298

来源：

EXPERIMENTAL CELL RESEARCH | 1991年 / 193卷 / 02期

关键词：

D O I：

10.1016/0014-4827(91)90113-9

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A modified method to synchronize CHO and HeLa cells is developed based upon a combined shaking-off and chemical blockage. This method has effectively blocked quiescient cells, which is the main obstacle of high degree synchronization. Flowcytometry data show the improvement on the degree of synchronization and yield compared to two previously used methods. © 1991.

引用

页码：405 / 410

页数：6

共 17 条

[1] NEW ANALYTICAL METHOD FOR DETERMINING DURATION OF PHASES, RATE OF DNA-SYNTHESIS AND DEGREE OF SYNCHRONIZATION FROM FLOW-CYTOMETRIC DATA ON SYNCHRONIZED CELL-POPULATIONS [J].